Emergence of Tetracycline Resistant Vibrio cholerae O1 Biotype El Tor Serotype Ogawa with Classical ctxB Gene from a Cholera Outbreak in Odisha, Eastern India.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2016-01-01 Epub Date: 2016-01-03 DOI:10.1155/2016/1695410

M Jain, P Kumar, A K Goel

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Abstract

In September 2010, a cholera outbreak was reported from Odisha, Eastern India. V. cholerae isolated from the clinical samples were biochemically and serologically confirmed as serogroup O1, biotype El Tor, and serotype Ogawa. Multiplex PCR screening revealed the presence of various genes, namely, ompW, ctxB, zot, rfbO1, tcp, ace, hlyA, ompU, rtx, and toxR, in all of the isolates. The isolates were resistant to co-trimoxazole, nalidixic acid, polymyxin B, spectinomycin, streptomycin, sulfamethoxazole, tetracycline, trimethoprim, and vibriostatic agent 2,4-diamino-6,7-diisopropylpteridine (O/129). Minimum inhibitory concentration of tetracycline decreased in the presence of carbonyl cyanide m-chlorophenylhydrazone (CCCP), suggesting the involvement of efflux pumps. PCR analysis confirmed the presence of class I integrons as well as SXT elements harbouring antibiotic resistance genes in all isolates. Sequencing revealed the presence of ctxB gene of classical biotype in all the isolates. The isolates harboured an RS1-CTX prophage array with El Tor type rstR and classical ctxB on the large chromosome. The study indicated that the V. cholerae El Tor variants are evolving in the area with better antibiotic resistance and virulence potential.

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印度东部奥迪沙爆发的霍乱疫情中出现了带有经典ctxB基因的耐四环素霍乱弧菌O1生物型El Tor血清型Ogawa。

2010 年 9 月，印度东部的奥迪沙爆发了霍乱疫情。从临床样本中分离出的霍乱弧菌经生化和血清学证实为 O1 血清群、El Tor 生物型和 Ogawa 血清型。多重聚合酶链式反应筛选显示，所有分离物中都含有多种基因，即 ompW、ctxB、zot、rfbO1、tcp、ace、hlyA、ompU、rtx 和 toxR。这些分离物对联合三唑、萘啶酸、多粘菌素 B、广谱霉素、链霉素、磺胺甲噁唑、四环素、三甲双胍和弧菌抑制剂 2,4-二氨基-6,7-二异丙基蝶啶（O/129）具有抗药性。四环素的最小抑制浓度在间氯苯基腙（CCCP）的作用下降低，这表明有外排泵的参与。PCR 分析证实，在所有分离物中都存在 I 类整合子和携带抗生素耐药基因的 SXT 元件。测序结果显示，所有分离物中都含有经典生物型的ctxB基因。这些分离物的大染色体上都有一个 RS1-CTX 原噬菌体阵列，其中包含 El Tor 型 rstR 和经典的 ctxB。该研究表明，霍乱弧菌 El Tor 变体正在该地区进化，具有更好的抗生素耐药性和毒力潜能。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.