Characterizing Wool Keratin

4区材料科学 Q2 Engineering

Advances in Materials Science and Engineering Pub Date : 2009-06-16 DOI:10.1155/2009/147175

J. M. Cardamone, A. Nuñez, Rafael A. Garcia, M. Aldema-Ramos

引用次数: 49

Abstract

Keratin from wool is a reactive, biocompatible, and biodegradable material. As the biological structural component of skin (soft keratins) and of nails, claws, hair, horn, feathers, and scales (hard keratins) pure keratin comprises up to 90% by weight of wool. Wool was treated in alkaline solutions to extract from 68% to 82% keratin within 2 to 5 hours of exposure at 6 5 ∘ C . The keratin products were water-soluble and were confirmed to contain intermediate filament and microfibrillar component-proteins of fractured, residual cuticle, and cortical cells. Oxidation of wool by peroxycarboximidic acid in alkaline hydrogen peroxide produced keratin products with distinct microcrystalline structures: descaled fibers, fibrous matrices, and lyophilized powders. Morphology and confirmation of peptide functionality were documented by SEM, Amino Acid Analysis, SDS-PAGE gel electrophoresis, MALDI-TOF/TOF, and FTIR analyses. The reactivity of keratin from wool models the reactivity of keratin from low-value sources such as cattle hair.

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羊毛角蛋白特征

羊毛角蛋白是一种活性物质，具有生物相容性和可生物降解性。作为皮肤(软角蛋白)和指甲、爪子、头发、角、羽毛和鳞片(硬角蛋白)的生物结构成分，纯角蛋白占羊毛重量的90%。羊毛在碱性溶液中处理，在65°C下暴露2至5小时内提取68%至82%的角蛋白。角蛋白产物是水溶性的，并被证实含有断裂、残余角质层和皮质细胞的中间纤维和微纤维成分。在碱性过氧化氢中，过氧羧酸氧化羊毛产生具有独特微晶结构的角蛋白产物:去鳞纤维、纤维基质和冻干粉末。通过扫描电镜、氨基酸分析、SDS-PAGE凝胶电泳、MALDI-TOF/TOF和FTIR分析记录了肽的形态和功能。羊毛角蛋白的反应性模拟了低价值来源(如牛毛)角蛋白的反应性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Advances in Materials Science and Engineering Materials Science-General Materials Science

CiteScore

3.30

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Advances in Materials Science and Engineering is a broad scope journal that publishes articles in all areas of materials science and engineering including, but not limited to: -Chemistry and fundamental properties of matter -Material synthesis, fabrication, manufacture, and processing -Magnetic, electrical, thermal, and optical properties of materials -Strength, durability, and mechanical behaviour of materials -Consideration of materials in structural design, modelling, and engineering -Green and renewable materials, and consideration of materials’ life cycles -Materials in specialist applications (such as medicine, energy, aerospace, and nanotechnology)