Early sum frequency generation vibrational spectroscopic studies on peptides and proteins at interfaces.

IF 1.6 4区医学 Q4 BIOPHYSICS

Biointerphases Pub Date : 2022-05-01 DOI:10.1116/6.0001859

Zhan Chen

引用次数: 6

Abstract

This paper summarizes the early research results on studying proteins and peptides at interfaces using sum frequency generation (SFG) vibrational spectroscopy. SFG studies in the C-H stretching frequency region to examine the protein side-chain behavior and in the amide I frequency region to investigate the orientation and conformation of interfacial peptides/proteins are presented. The early chiral SFG research and SFG isotope labeling studies on interfacial peptides/proteins are also discussed. These early SFG studies demonstrate the feasibility of using SFG to elucidate interfacial molecular structures of peptides and proteins in situ, which built a foundation for later SFG investigations on peptides and proteins at interfaces.

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多肽和蛋白质界面处的早期和频产生振动光谱研究。

本文综述了用和频产生(SFG)振动光谱技术研究蛋白质和多肽界面的早期研究成果。在C-H拉伸频率区研究蛋白质侧链行为，在酰胺I频率区研究界面肽/蛋白质的取向和构象，提出了SFG研究。讨论了早期手性SFG研究和界面肽/蛋白的SFG同位素标记研究。这些早期的SFG研究证明了利用SFG原位阐明多肽和蛋白质界面分子结构的可行性，为后来在界面上研究多肽和蛋白质的SFG奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biointerphases 生物-材料科学：生物材料

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期刊介绍： Biointerphases emphasizes quantitative characterization of biomaterials and biological interfaces. As an interdisciplinary journal, a strong foundation of chemistry, physics, biology, engineering, theory, and/or modelling is incorporated into originated articles, reviews, and opinionated essays. In addition to regular submissions, the journal regularly features In Focus sections, targeted on specific topics and edited by experts in the field. Biointerphases is an international journal with excellence in scientific peer-review. Biointerphases is indexed in PubMed and the Science Citation Index (Clarivate Analytics). Accepted papers appear online immediately after proof processing and are uploaded to key citation sources daily. The journal is based on a mixed subscription and open-access model: Typically, authors can publish without any page charges but if the authors wish to publish open access, they can do so for a modest fee. Topics include: bio-surface modification nano-bio interface protein-surface interactions cell-surface interactions in vivo and in vitro systems biofilms / biofouling biosensors / biodiagnostics bio on a chip coatings interface spectroscopy biotribology / biorheology molecular recognition ambient diagnostic methods interface modelling adhesion phenomena.