Injection of Propidium Iodide into HeLa Cells Using a Silicon Nanoinjection Lance Array

Zachary K. Lindstrom, Steven J. Brewer, M. A. Ferguson, S. Burnett, B. Jensen
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引用次数: 10

Abstract

Delivering foreign molecules into human cells is a wide and ongoing area of research. Gene therapy, or delivering nucleic acids into cells via nonviral or viral pathways, is an especially promising area for pharmaceutics. All gene therapy methods have their respective advantages and disadvantages, including limited delivery efficiency and low viability. We present an electromechanical method for delivering foreign molecules into human cells. Nanoinjection, or delivering molecules into cells using a solid lance, has proven to be highly efficient while maintaining high viability levels. This paper describes an array of solid silicon microlances that was tested to determine efficiency and viability when nanoinjecting tens of thousands of HeLa cells simultaneously. Propidium iodide (PI), a dye that fluoresces when bound to nucleic acids and does not fluoresce when unbound, was delivered into cells using the lance array. Results show that the lance array delivers PI into up to 78 % of a nanoinjected HeLa cell culture, while maintaining 78–91 % viability. With these results, we submit the nanoinjection method using a silicon lance array as another promising particle delivery method for mammalian culture cells. [DOI: 10.1115/1.4028603]
利用硅纳米注射枪阵列向HeLa细胞注射碘化丙啶
将外来分子输送到人体细胞中是一个广泛而持续的研究领域。基因治疗,或通过非病毒或病毒途径将核酸输送到细胞中,是制药领域一个特别有前途的领域。所有的基因治疗方法都有各自的优点和缺点,包括递送效率有限和生存力低。我们提出了一种将外来分子输送到人体细胞中的机电方法。纳米注射,或使用固体喷枪将分子输送到细胞中,已被证明是高效的,同时保持高活力水平。本文介绍了一种固体硅微枪阵列,该阵列在同时注入数万个HeLa细胞时进行了测试,以确定其效率和活力。碘化丙啶(PI)是一种与核酸结合时发出荧光而非结合时不发出荧光的染料,使用喷枪阵列将其送入细胞。结果表明,喷枪阵列将PI输送到高达78%的纳米注射HeLa细胞培养物中,同时保持78 - 91%的存活率。基于这些结果,我们提出了硅枪阵列纳米注射方法作为哺乳动物培养细胞的另一种有前途的颗粒递送方法。(DOI: 10.1115/1.4028603)
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