Se-Eun Park, Hyo-Jeong Lee, In-Seon Jeong, Seung Kim
{"title":"Mesembryanthemum crystallinum extract ameliorates\n TNF-α-induced inflammation and insulin\n resistance in 3T3-L1 adipocytes","authors":"Se-Eun Park, Hyo-Jeong Lee, In-Seon Jeong, Seung Kim","doi":"10.11002/kjfp.2022.29.6.1000","DOIUrl":null,"url":null,"abstract":"\n \n Obesity is classified as a low-grade chronic inflammatory disease.\n Obesity-induced inflammation is associated with the development of insulin\n resistance (IR). In this study, we investigated the effects of\n Mesembryanthemum crystallinum extract (MCE) on tumor\n necrosis factor-alpha (TNF-α)-induced inflammation and\n IR in 3T3-L1 adipocytes, as well as underlying mechanisms. Lipolysis was\n monitored by oil red O staining. Glucose uptake was measured by 2-deoxyglucose\n (2-DG). The production of cytokines and adiponectin was evaluated using\n enzyme-linked immunosorbent assay (ELISA). Protein expression was measured using\n western blotting. IR was induced in differentiated 3T3-L1 adipocytes by\n treatment with TNF-α. MCE treatment decreased lipolysis\n while increasing adiponectin production. MCE inhibited the production of\n pro-inflammatory cytokines such as interleukn (IL)-6 and monocyte\n chemoattractant protein (MCP)-1, as well as the Nuclear\n factor-κB (NF-κB)\n translocation. Additionally, MCE increased glucose uptake, prevented the protein\n expression of phosphorylated insulin receptor supstrate (IRS) and increased the\n protein expression of Glucose Transporter Type 4 (GLUT4) in a dose-dependent\n manner. These results demonstrate the potential of MCE in improving chronic\n inflammation and insulin sensitivity in obesity. Therefore, MCE can be used as a\n novel candidate for developing functional foods.\n","PeriodicalId":17875,"journal":{"name":"Korean Journal of Food Preservation","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Korean Journal of Food Preservation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.11002/kjfp.2022.29.6.1000","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0
Abstract
Obesity is classified as a low-grade chronic inflammatory disease.
Obesity-induced inflammation is associated with the development of insulin
resistance (IR). In this study, we investigated the effects of
Mesembryanthemum crystallinum extract (MCE) on tumor
necrosis factor-alpha (TNF-α)-induced inflammation and
IR in 3T3-L1 adipocytes, as well as underlying mechanisms. Lipolysis was
monitored by oil red O staining. Glucose uptake was measured by 2-deoxyglucose
(2-DG). The production of cytokines and adiponectin was evaluated using
enzyme-linked immunosorbent assay (ELISA). Protein expression was measured using
western blotting. IR was induced in differentiated 3T3-L1 adipocytes by
treatment with TNF-α. MCE treatment decreased lipolysis
while increasing adiponectin production. MCE inhibited the production of
pro-inflammatory cytokines such as interleukn (IL)-6 and monocyte
chemoattractant protein (MCP)-1, as well as the Nuclear
factor-κB (NF-κB)
translocation. Additionally, MCE increased glucose uptake, prevented the protein
expression of phosphorylated insulin receptor supstrate (IRS) and increased the
protein expression of Glucose Transporter Type 4 (GLUT4) in a dose-dependent
manner. These results demonstrate the potential of MCE in improving chronic
inflammation and insulin sensitivity in obesity. Therefore, MCE can be used as a
novel candidate for developing functional foods.
期刊介绍:
This journal aims to promote and encourage the advancement of quantitative improvement for the storage, processing and distribution of food and its related disciplines, theory and research on its application. Topics covered include: Food Preservation and Packaging Food and Food Material distribution Fresh-cut Food Manufacturing Food processing Technology Food Functional Properties Food Quality / Safety.