Effect of in vitro ribosylation on the dynamic fracture behavior of mature bovine cortical bone

IF 3.3 2区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of the Mechanical Behavior of Biomedical Materials Pub Date : 2023-10-17 DOI:10.1016/j.jmbbm.2023.106171

Tanner Snow , William Woolley , Claire Acevedo , Owen T. Kingstedt

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引用次数: 0

Abstract

In this study, the fracture behavior of ribosylated bovine cortical bone is investigated under loading conditions simulating a fall event. Single edge notched specimens, separated into a control group (n = 11) and a ribosylated group (n = 8), were extracted from the mid-diaphysis of a single bovine femur harvested from a mature cow. A seven-day ribosylation process results in the accumulation of Advanced-Glycation End Products (AGEs) cross-links and AGE adducts. Specimens were subjected to symmetric three point bending (opening mode) and an impact velocity of 1.6 m/s using a drop tower. Near-crack displacement fields up to fracture initiation are determined from high-speed images post-processed using digital image correlation. A constrained over-deterministic least squares regression and orthotropic material linear elastic fracture mechanics theory are used to extract the in-plane critical stress intensity factors at fracture initiation (i.e., fracture initiation toughness values). Statistically significant differences were not observed when comparing the in-plane fracture initiation toughness values ( $p \geq 0.96$ ) or energy release rate ( $p = 0.90$ ) between the control and seven-day ribosylated groups. The intrinsic variability of bone may require high sample numbers in order to achieve an adequately powered experiment when assessing dynamic fracture behavior. While there are no detectable differences due to the ribosylation treatment investigated, this is likely due to the limited sample sizes utilized.

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体外核糖基化对成熟牛皮质骨动态断裂行为的影响。

在本研究中，研究了核糖基化牛皮质骨在模拟跌倒事件的负载条件下的骨折行为。从成熟奶牛的单个牛股骨的中骨干中提取单侧切口标本，分为对照组（n=11）和核糖基化组（n=8）。七天的核糖基化过程导致晚期糖基化终产物（AGEs）交联和AGE加合物的积累。使用跌落塔对试样进行对称三点弯曲（打开模式）和1.6m/s的冲击速度。从使用数字图像相关进行后处理的高速图像中确定直至断裂萌生的近裂纹位移场。使用约束超确定性最小二乘回归和正交各向异性材料线弹性断裂力学理论来提取断裂起始时的面内临界应力强度因子（即断裂起始韧性值）。当比较对照组和七天核糖基化组之间的平面内断裂起始韧性值（p≥0.96）或能量释放率（p=0.90）时，没有观察到统计学上的显著差异。骨骼的内在可变性可能需要高样本数量，以便在评估动态骨折行为时实现充分的实验。虽然由于所研究的核糖基化处理没有可检测的差异，但这可能是由于所使用的样本量有限。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Mechanical Behavior of Biomedical Materials 工程技术-材料科学：生物材料

CiteScore

7.20

自引率

7.70%

发文量

505

审稿时长

46 days

期刊介绍： The Journal of the Mechanical Behavior of Biomedical Materials is concerned with the mechanical deformation, damage and failure under applied forces, of biological material (at the tissue, cellular and molecular levels) and of biomaterials, i.e. those materials which are designed to mimic or replace biological materials. The primary focus of the journal is the synthesis of materials science, biology, and medical and dental science. Reports of fundamental scientific investigations are welcome, as are articles concerned with the practical application of materials in medical devices. Both experimental and theoretical work is of interest; theoretical papers will normally include comparison of predictions with experimental data, though we recognize that this may not always be appropriate. The journal also publishes technical notes concerned with emerging experimental or theoretical techniques, letters to the editor and, by invitation, review articles and papers describing existing techniques for the benefit of an interdisciplinary readership.