Reverse pharmacology approach to validate the diabetic wound-healing activity of Jatyadi thailam formulations in vitro on diabetic mimic environment.

IF 2.5 4区医学 Q3 ENDOCRINOLOGY & METABOLISM

Archives of Physiology and Biochemistry Pub Date : 2023-10-28 DOI:10.1080/13813455.2023.2264536

Kandasamy Swathi, Sundaravadivelu Sumathi, Kumar Somit, Sripathi K Shubashini

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引用次数: 0

Abstract

Objective: Jatyadi thailam, an Ayurvedic preparation, is renowned for its efficacy in diabetic wound healing and inflammation. This study aimed to validate and compare the diabetic wound-healing potential of two Jatyadi thailam formulations - Ayurvedic formulary of India Jatyadi thailam (JT-AFI) and Yogagrantha formulation of Jatyadi thailam (JT-YG), in a diabetic environment using L929 fibroblast cells in vitro. Methodology: The effects on cell survival, proliferation, migration, angiogenesis, cell cycle progression, apoptosis, ROS generation, and mitochondrial function were evaluated.Results: The formulations promoted cell proliferation, migration, angiogenesis, while also regulating cell cycle and apoptosis. They effectively suppressed ROS generation and modulated mitochondrial function. JT-AFI exhibited superior efficacy in accelerating diabetic wound healing compared to JT-YG.Conclusion: These findings provide substantial support for the mechanistic role of Jatyadi thailam in diabetic wound healing.

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反向药理学方法验证Jatyadi thailam制剂在糖尿病模拟环境中的糖尿病伤口愈合活性。

目的：阿育吠陀制剂Jatyadi thailam以其对糖尿病伤口愈合和炎症的疗效而闻名。本研究旨在使用L929成纤维细胞体外验证和比较两种Jatyadi thailam配方——印度阿育吠陀配方Jatyadi-thailam（JT-AFI）和Jatyadi-thailam的Yogagrantha配方（JT-YG）——在糖尿病环境中的糖尿病伤口愈合潜力。方法：评估对细胞存活、增殖、迁移、血管生成、细胞周期进展、细胞凋亡、ROS生成和线粒体功能的影响。结果：该制剂促进细胞增殖、迁移、血管生成，同时调节细胞周期和凋亡。它们有效抑制ROS的产生并调节线粒体功能。与JT-YG相比，JT-AFI在加速糖尿病伤口愈合方面表现出优异的疗效。结论：这些发现为Jatyadi thailam在糖尿病伤口愈合中的机制作用提供了实质性支持。

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来源期刊

Archives of Physiology and Biochemistry ENDOCRINOLOGY & METABOLISM-PHYSIOLOGY

CiteScore

6.90

自引率

3.30%

发文量

期刊介绍： Archives of Physiology and Biochemistry: The Journal of Metabolic Diseases is an international peer-reviewed journal which has been relaunched to meet the increasing demand for integrated publication on molecular, biochemical and cellular aspects of metabolic diseases, as well as clinical and therapeutic strategies for their treatment. It publishes full-length original articles, rapid papers, reviews and mini-reviews on selected topics. It is the overall goal of the journal to disseminate novel approaches to an improved understanding of major metabolic disorders. The scope encompasses all topics related to the molecular and cellular pathophysiology of metabolic diseases like obesity, type 2 diabetes and the metabolic syndrome, and their associated complications. Clinical studies are considered as an integral part of the Journal and should be related to one of the following topics: -Dysregulation of hormone receptors and signal transduction -Contribution of gene variants and gene regulatory processes -Impairment of intermediary metabolism at the cellular level -Secretion and metabolism of peptides and other factors that mediate cellular crosstalk -Therapeutic strategies for managing metabolic diseases Special issues dedicated to topics in the field will be published regularly.