Facilitating In Situ Tumor Imaging with a Tetrahedral DNA Framework-Enhanced Hybridization Chain Reaction Probe

IF 19.9 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Advanced Functional Materials Pub Date : 2022-01-06 DOI:10.1002/adfm.202109728

Bowen Zhang, Taoran Tian, Dexuan Xiao, Shaojingya Gao, Xiaoxiao Cai, Yunfeng Lin

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引用次数: 74

Abstract

Rapid and efficient tools for early cancer detection have diagnostic and therapeutic value. Given that the DNA hairpin‐based hybridization chain reaction (HCR) is effective in detecting various biological targets, a tetrahedral framework DNA‐enhanced (TDN‐enhanced) HCR detection system (T‐probe system) is introduced for cancer‐related targets and its versatility is demonstrated by detecting intracellular target miRNA 21 and cellular membrane target nucleolin. Benefiting from the spatial confinement of the TDN, the T‐probe system demonstrates a high detection rate. It increases the reaction efficiency of nude hairpins in vitro while accurately and rapidly recognizing both membrane and intracellular cancer‐related targets in living cells. Furthermore, it exhibits superior fluorescence in vivo within 15 s of peripheral‐tumor injection and 10 min of tail‐vein injection. The T‐probe system efficiently and accurately images tumor‐related biomarkers in vitro and in vivo, thereby demonstrating great potential for clinical diagnosis and therapeutic applications.

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利用四面体DNA框架增强杂交链反应探针促进原位肿瘤成像

快速有效的癌症早期检测工具具有诊断和治疗价值。鉴于基于DNA发夹的杂交链反应(HCR)可有效检测多种生物靶点，本文介绍了一种针对癌症相关靶点的四面体框架DNA增强(TDN-enhanced) HCR检测系统(T-probe system)，并通过检测细胞内靶点miRNA 21和细胞膜靶点核蛋白证明了其多功能性。得益于TDN的空间限制，t探针系统具有较高的检测率。它提高了裸发夹在体外的反应效率，同时准确、快速地识别活细胞中的膜和细胞内癌症相关靶点。在肿瘤外周注射15 s和尾静脉注射10 min内，在体内表现出优越的荧光性。t探针系统在体外和体内高效准确地成像肿瘤相关生物标志物，从而显示出临床诊断和治疗应用的巨大潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Advanced Functional Materials 工程技术-材料科学：综合

CiteScore

29.50

自引率

4.20%

发文量

2086

审稿时长

2.1 months

期刊介绍： Firmly established as a top-tier materials science journal, Advanced Functional Materials reports breakthrough research in all aspects of materials science, including nanotechnology, chemistry, physics, and biology every week. Advanced Functional Materials is known for its rapid and fair peer review, quality content, and high impact, making it the first choice of the international materials science community.