RAD51C-RAD51D interplays with MSH5 and regulates crossover maturation in rice meiosis

IF 9.4 1区生物学 Q1 Agricultural and Biological Sciences

New Phytologist Pub Date : 2023-07-10 DOI:10.1111/nph.19095

Fanfan Zhang, Wenqing Shi, Yue Zhou, Lijun Ma, Yongjie Miao, Na Mu, Haiyun Ren, Zhukuan Cheng

{"title":"RAD51C-RAD51D interplays with MSH5 and regulates crossover maturation in rice meiosis","authors":"Fanfan Zhang, Wenqing Shi, Yue Zhou, Lijun Ma, Yongjie Miao, Na Mu, Haiyun Ren, Zhukuan Cheng","doi":"10.1111/nph.19095","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n \n <ul>\n \n \n <li>Meiotic crossovers ensure accurate chromosome segregation and increase genetic diversity. RAD51C and RAD51D play an early role in facilitating RAD51 during homologous recombination. However, their later function in meiosis is largely unknown in plants.</li>\n \n \n <li>Here, through targeted disruption of RAD51C and RAD51D, we generated three new mutants and revealed their later meiotic role in crossover maturation.</li>\n \n \n <li>The rad51c-3 and rad51d-4 mutants showed a mixture of bivalents and univalents and no chromosomal entanglements, whereas rad51d-5 exhibited an intermediate phenotype with reduced chromosomal entanglements and increased bivalent formation compared with knockout alleles. Comparisons of RAD51 loadings and chromosomal entanglements in these single mutants, rad51c-3 rad51d-4, rad51c-3 dmc1a dmc1b, and rad51d-4 dmc1a dmc1b suggest that the retained level of RAD51 in mutants is required for uncovering their function in crossover formation. Reductions in chiasma frequency and later HEI10 foci in these mutants support that crossover maturation requires RAD51C and RAD51D. Moreover, interaction between RAD51D and MSH5 indicates that RAD51 paralogs may cooperate with MSH5 to ensure accurate Holliday junction processing into crossover products.</li>\n \n \n <li>This finding of the role of RAD51 paralogs in crossover control may be conserved from mammals to plants and advances our current understanding of these proteins.</li>\n </ul>\n \n </div>","PeriodicalId":48887,"journal":{"name":"New Phytologist","volume":"239 5","pages":"1790-1803"},"PeriodicalIF":9.4000,"publicationDate":"2023-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"New Phytologist","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/nph.19095","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}

引用次数: 0

Abstract

Meiotic crossovers ensure accurate chromosome segregation and increase genetic diversity. RAD51C and RAD51D play an early role in facilitating RAD51 during homologous recombination. However, their later function in meiosis is largely unknown in plants.
Here, through targeted disruption of RAD51C and RAD51D, we generated three new mutants and revealed their later meiotic role in crossover maturation.
The rad51c-3 and rad51d-4 mutants showed a mixture of bivalents and univalents and no chromosomal entanglements, whereas rad51d-5 exhibited an intermediate phenotype with reduced chromosomal entanglements and increased bivalent formation compared with knockout alleles. Comparisons of RAD51 loadings and chromosomal entanglements in these single mutants, rad51c-3 rad51d-4, rad51c-3 dmc1a dmc1b, and rad51d-4 dmc1a dmc1b suggest that the retained level of RAD51 in mutants is required for uncovering their function in crossover formation. Reductions in chiasma frequency and later HEI10 foci in these mutants support that crossover maturation requires RAD51C and RAD51D. Moreover, interaction between RAD51D and MSH5 indicates that RAD51 paralogs may cooperate with MSH5 to ensure accurate Holliday junction processing into crossover products.
This finding of the role of RAD51 paralogs in crossover control may be conserved from mammals to plants and advances our current understanding of these proteins.

查看原文本刊更多论文

RAD51C-RAD51D与MSH5相互作用，调控水稻减数分裂的交叉成熟

减数分裂交叉确保准确的染色体分离和增加遗传多样性。在同源重组过程中，RAD51C和RAD51D在促进RAD51的早期发挥作用。然而，它们在植物减数分裂中的后期功能在很大程度上是未知的。在这里，我们通过靶向破坏RAD51C和RAD51D，产生了三个新的突变体，并揭示了它们在交叉成熟中的减数分裂作用。与敲除等位基因相比，rad51c-3和rad51d-4突变体表现出二价和一价的混合物，没有染色体纠缠，而rad51d-5表现出一种中间表型，染色体纠缠减少，二价形成增加。rad51c-3、rad51d-4、rad51c-3 dmc1a、dmc1b和rad51d-4 dmc1a、dmc1b中rad51c-3的装载量和染色体纠缠的比较表明，rad51d-4 dmc1a、dmc1b中rad51a的保留水平是揭示它们在交叉形成中的功能所必需的。这些突变体中交叉频率的减少和后来的he10病灶支持交叉成熟需要RAD51C和RAD51D。此外，RAD51D与MSH5之间的相互作用表明，RAD51平行线可以与MSH5合作，以确保精确的Holliday结加工成交叉产品。RAD51类似物在交叉控制中的作用的这一发现可能从哺乳动物到植物都是保守的，并推进了我们目前对这些蛋白质的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

New Phytologist PLANT SCIENCES-

CiteScore

17.60

自引率

5.30%

发文量

728

审稿时长

1 months

期刊介绍： New Phytologist is a leading publication that showcases exceptional and groundbreaking research in plant science and its practical applications. With a focus on five distinct sections - Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology - the journal covers a wide array of topics ranging from cellular processes to the impact of global environmental changes. We encourage the use of interdisciplinary approaches, and our content is structured to reflect this. Our journal acknowledges the diverse techniques employed in plant science, including molecular and cell biology, functional genomics, modeling, and system-based approaches, across various subfields.