The RECEPTOR-LIKE PROTEIN53 immune complex associates with LLG1 to positively regulate plant immunity

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Renjie Chen, Pengwei Sun, Guitao Zhong, Wei Wang, Dingzhong Tang
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引用次数: 4

Abstract

Pattern recognition receptors (PRRs) sense ligands in pattern-triggered immunity (PTI). Plant PRRs include numerous receptor-like proteins (RLPs), but many RLPs remain functionally uncharacterized. Here, we examine an Arabidopsis thaliana RLP, RLP53, which positively regulates immune signaling. Our forward genetic screen for suppressors of enhanced disease resistance1 (edr1) identified a point mutation in RLP53 that fully suppresses disease resistance and mildew-induced cell death in edr1 mutants. The rlp53 mutants showed enhanced susceptibility to virulent pathogens, including fungi, oomycetes, and bacteria, indicating that RLP53 is important for plant immunity. The ectodomain of RLP53 contains leucine-rich repeat (LRR) motifs. RLP53 constitutively associates with the LRR receptor-like kinase SUPPRESSOR OF BRASSINOSTEROID INSENSITIVE1-ASSOCIATED KINASE (BAK1)-INTERACTING RECEPTOR KINASE1 (SOBIR1) and interacts with the co-receptor BAK1 in a pathogen-induced manner. The double mutation sobir1-12 bak1-5 suppresses edr1-mediated disease resistance, suggesting that EDR1 negatively regulates PTI modulated by the RLP53–SOBIR1–BAK1 complex. Moreover, the glycosylphosphatidylinositol (GPI)-anchored protein LORELEI-LIKE GPI-ANCHORED PROTEIN1 (LLG1) interacts with RLP53 and mediates RLP53 accumulation in the plasma membrane. We thus uncovered the role of a novel RLP and its associated immune complex in plant defense responses and revealed a potential new mechanism underlying regulation of RLP immune function by a GPI-anchored protein.

Abstract Image

受体样蛋白53免疫复合物与LLG1结合,积极调节植物免疫
模式识别受体(PRRs)在模式触发免疫(PTI)中的感觉配体。植物PRRs包括许多受体样蛋白(rlp),但许多rlp仍未被功能表征。在这里,我们研究了拟南芥RLP, RLP53,它积极调节免疫信号。我们对增强疾病抗性1 (edr1)抑制因子的正向遗传筛选发现了RLP53中的一个点突变,该突变完全抑制edr1突变体的疾病抗性和霉菌诱导的细胞死亡。rlp53突变体对毒力病原体(包括真菌、卵菌和细菌)的易感性增强,表明rlp53对植物免疫很重要。RLP53的外结构域含有富含亮氨酸的重复序列(LRR)基序。RLP53组成性地与油菜素内酯不敏感相关激酶(BAK1)-相互作用受体KINASE1 (SOBIR1)的LRR受体样激酶抑制因子结合,并以病原体诱导的方式与共受体BAK1相互作用。双突变sobir1-12 bak1-5抑制EDR1介导的疾病抗性,表明EDR1负性调节RLP53-SOBIR1-BAK1复合物调控的PTI。此外,糖基磷脂酰肌醇(GPI)锚定蛋白LORELEI-LIKE GPI-锚定蛋白1 (LLG1)与RLP53相互作用并介导RLP53在质膜中的积累。因此,我们揭示了一种新的RLP及其相关免疫复合物在植物防御反应中的作用,并揭示了gpi锚定蛋白调控RLP免疫功能的潜在新机制。
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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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