Fabrication and evaluation of multifunctional agarose based electrospun scaffolds for cutaneous wound repairs

Abstract

Despite several advances in chronic wound management, natural product based scaffolds with high exude absorption and mechanical strength are still a hotspot in the medical field. Thus, present study illustrates the fabrication of agarose (AG; 10% w/v)/polyvinyl alcohol 12% w/v) based multifunctional nanofibrous electrospun scaffolds. Zinc citrate (1%, 3% and 5% w/w of the polymer) was used as a potential antibacterial agent. The fabricated scaffolds exhibit a swelling of ∼550% in phosphate buffer saline and mechanical strength of 10.11 ± 0.31 MPa which is suitable for most of the wound healing applications that require high strength. In vitro study revealed an increased migration and proliferation of L929 fibroblasts with AG blends when compared to the control. The fabricated scaffolds exhibited antibacterial properties against both Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative) bacterial strains. Hence, a multifunctional (ability to protect wounds from bacterial infections along with effective swelling and mechanical support), natural product based, eco-friendly scaffold to serve as a potential wound dressing material has been successfully fabricated.