Histological and immunohistochemical study of the potential therapeutic impacts of bone marrow mesenchymal stem cells and exosomes for sciatic nerve crush injury model in rats

IF 0.6 4区生物学 Q4 CELL BIOLOGY

Journal of Histotechnology Pub Date : 2018-09-15 DOI:10.1080/01478885.2018.1505205

N. El-Azab, Abeer M El-Mahalaway, O. Mostafa, D. Sabry

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引用次数: 7

Abstract

ABSTRACT The aim was to evaluate the potential effect of bone marrow-derived mesenchymal stem cells (BMSC) and BMSC-EX in the sciatic nerve injury. Forty-five rats were divided into four groups, i.e. Group I control no surgery or treatment, Group II sciatic nerve crush surgery and 1 week post-surgery, Group III sciatic nerve crush injury was treated with BMSC, and Group IV sciatic nerve crush injury was treated with BMSC-EX. All animal groups were euthanized 2 weeks after treatment. Sciatic nerves were examined histologically and immunohistochemically. Group II showed various histological changes, i.e. vacuolation, degeneration and loss of nerve fibers. Group II showed a significant increase in collagen fibers, but with significant reduction in osmium tetroxide (OsO4)-stained myelin sheaths and neurofilament immunostaining compared to Group I. Group II also revealed distortion and disruption of myelinated nerve fibers. Groups III and IV showed significant improvement of both histological and immunohistochemical changes in Group II. BMSC and BMSC-EX were shown to improve axonal regeneration.

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骨髓间充质干细胞和外泌体对大鼠坐骨神经挤压损伤模型潜在治疗作用的组织学和免疫组织化学研究

目的是评估骨髓间充质干细胞(BMSC)和骨髓间充质干细胞- ex在坐骨神经损伤中的潜在作用。将45只大鼠分为4组，ⅰ组对照组不手术或治疗，ⅱ组坐骨神经压伤术后1周，ⅲ组坐骨神经压伤后给予BMSC治疗，ⅳ组坐骨神经压伤后给予BMSC- ex治疗。各组动物均于治疗2周后安乐死。对坐骨神经进行组织学和免疫组织化学检查。II组表现为各种组织学改变，如空泡化、变性和神经纤维丢失。与i组相比，II组胶原纤维明显增加，但四氧化锇(OsO4)染色的髓鞘和神经丝免疫染色明显减少。II组还显示有髓神经纤维扭曲和破坏。III组和IV组的组织学和免疫组化变化在II组均有明显改善。BMSC和BMSC- ex可促进轴突再生。

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来源期刊

Journal of Histotechnology 生物-细胞生物学

CiteScore

2.60

自引率

9.10%

发文量

审稿时长

>12 weeks

期刊介绍： The official journal of the National Society for Histotechnology, Journal of Histotechnology, aims to advance the understanding of complex biological systems and improve patient care by applying histotechniques to diagnose, prevent and treat diseases. Journal of Histotechnology is concerned with educating practitioners and researchers from diverse disciplines about the methods used to prepare tissues and cell types, from all species, for microscopic examination. This is especially relevant to Histotechnicians. Journal of Histotechnology welcomes research addressing new, improved, or traditional techniques for tissue and cell preparation. This includes review articles, original articles, technical notes, case studies, advances in technology, and letters to editors. Topics may include, but are not limited to, discussion of clinical, veterinary, and research histopathology.