Auxin-mediated regulation of arbuscular mycorrhizal symbiosis: A role of SlGH3.4 in tomato

IF 5.3 2区材料科学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY

ACS Applied Nano Materials Pub Date : 2021-10-29 DOI:10.1111/pce.14210

Xiao Chen, Jiadong Chen, Dehua Liao, Hanghang Ye, Cai Li, Zhenzhen Luo, Anning Yan, Qingchun Zhao, Kun Xie, Yiting Li, Dongsheng Wang, Jun Chen, Aiqun Chen, Guohua Xu

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引用次数: 14

Abstract

Most land plants can establish symbiosis with arbuscular mycorrhizal (AM) fungi to increase fitness to environmental challenges. The development of AM symbiosis is controlled by intricate procedures involving all phytohormones. However, the mechanisms underlying the auxin-mediated regulation of AM symbiosis remains largely unknown. Here, we report that AM colonisation promotes auxin response and indole-3-acetic acid (IAA) accumulation, but downregulates IAA biosynthesis genes in tomato (Solanum lycopersicum). External IAA application modulates the AM symbiosis by promoting arbuscule formation at low concentrations but repressing it at high concentrations. An AM-induced GH3 gene, SlGH3.4, encoding a putative IAA-amido synthetase, negatively regulates mycorrhization via maintaining cellular auxin homoeostasis. Loss of SlGH3.4 function increased free IAA content and arbuscule incidence, while constitutively overexpressing SlGH3.4 in either tomato or rice resulted in decreased IAA content, total colonisation level and arbuscule abundance in mycorrhizal roots. Several auxin-inducible expansin genes involved in AM formation or resistance to pathogen infection were upregulated in slgh3.4 mycorrhizal roots but downregulated in the SlGH3.4-overexpressing plants. Taken together, our results highlight a positive correlation between the endogenous IAA content and mycorrhization level, particularly arbuscule incidence, and suggest that the SlGH3.4-mediated auxin homoeostasis and regulation of expansin genes is involved in finely tuning the AM development.

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生长素介导的番茄丛枝菌根共生调节:SlGH3.4的作用

大多数陆地植物可以与丛枝菌根(AM)真菌建立共生关系，以增加对环境挑战的适应性。AM共生的发展是由涉及所有植物激素的复杂过程控制的。然而，生长素介导的AM共生调节机制在很大程度上仍然未知。在此，我们报道了AM定殖促进番茄(Solanum lycopersicum)生长素反应和IAA积累，但下调IAA生物合成基因。外源IAA通过在低浓度下促进丛枝形成而在高浓度下抑制丛枝形成来调节AM共生。am诱导的GH3基因SlGH3.4编码一种假定的iaa -氨基合成酶，通过维持细胞生长素平衡负调控菌根化。SlGH3.4功能缺失增加了游离IAA含量和丛枝发生率，而在番茄和水稻中组成性过表达SlGH3.4导致菌根中IAA含量、总定殖水平和丛枝丰度降低。一些生长素诱导的扩展蛋白基因参与AM的形成或抵抗病原体感染，在slgh3.4菌根中表达上调，而在slgh3.4过表达的植物中表达下调。综上所述，我们的研究结果突出了内源IAA含量与菌根化水平，特别是丛生菌发生率之间的正相关关系，并表明slgh3.4介导的生长素稳态和扩张蛋白基因的调控参与了AM发育的精细调节。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Nano Materials Multiple-

CiteScore

8.30

自引率

3.40%

发文量

1601

期刊介绍： ACS Applied Nano Materials is an interdisciplinary journal publishing original research covering all aspects of engineering, chemistry, physics and biology relevant to applications of nanomaterials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important applications of nanomaterials.