Three-dimensional imaging for the analysis of human epidermal melanocytes

IF 3.9 3区医学 Q2 CELL BIOLOGY

Pigment Cell & Melanoma Research Pub Date : 2022-05-27 DOI:10.1111/pcmr.13051

Dai Hyun Kim, Se Jeong Lee, Soo Hong Seo, Hyo Hyun Ahn, Byung-Jo Kim, Woong Sun, Im Joo Rhyu

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Abstract

Three-dimensional (3-D) analysis of human epidermal melanocytes is required for deeper understanding of melanocytic disorders. The purpose of this study was to standardize 3-D imaging and quantification for the evaluation of epidermal melanocytes. The epidermal specimen was obtained using the suction blister method from a patient with melanocytic nevus on the forearm skin. Cutaneous ACT-PRESTO, the tissue-clearing and labeling technique, was subsequently performed. With the 3-D image analysis program, morphological reconstruction and quantification of selected perilesional and melanocytic nevus areas were possible. The region of melanocytic nevus showed higher numbers of total melanocytic dendrites and similar numbers of cell bodies compared with perilesional area. In addition, the mean area and volume of cell bodies increased in the melanocytic nevus area compared with the results in the perilesional area. The 3-D evaluation method of human epidermal melanocytes can be applied to investigate novel pathologies related to hyper- or hypo-pigmentary disorders.

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用于分析人表皮黑色素细胞的三维成像

为了更深入地了解黑色素细胞疾病，需要对人类表皮黑色素细胞进行三维(3-D)分析。本研究的目的是标准化表皮黑色素细胞的三维成像和定量评估。采用吸泡法从前臂皮肤黑素细胞痣患者处获得表皮标本。随后进行皮肤ACT-PRESTO，组织清除和标记技术。使用三维图像分析程序，可以对选定的病灶周围和黑素细胞痣区域进行形态学重建和量化。黑素细胞痣区域的总黑素细胞树突和细胞体数量均高于病灶周围区域。此外，黑素细胞痣区域的平均面积和细胞体体积比病灶周围区域的结果增加。人类表皮黑色素细胞的三维评价方法可用于研究与色素过多或过少疾病相关的新病理。

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来源期刊

Pigment Cell & Melanoma Research 医学-皮肤病学

CiteScore

8.90

自引率

2.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Pigment Cell & Melanoma Researchpublishes manuscripts on all aspects of pigment cells including development, cell and molecular biology, genetics, diseases of pigment cells including melanoma. Papers that provide insights into the causes and progression of melanoma including the process of metastasis and invasion, proliferation, senescence, apoptosis or gene regulation are especially welcome, as are papers that use the melanocyte system to answer questions of general biological relevance. Papers that are purely descriptive or make only minor advances to our knowledge of pigment cells or melanoma in particular are not suitable for this journal. Keywords Pigment Cell & Melanoma Research, cell biology, melatonin, biochemistry, chemistry, comparative biology, dermatology, developmental biology, genetics, hormones, intracellular signalling, melanoma, molecular biology, ocular and extracutaneous melanin, pharmacology, photobiology, physics, pigmentary disorders