Single-Lens Light-Sheet Fluorescence Microscopy Based on Micro-Mirror Array

IF 10 1区 物理与天体物理 Q1 OPTICS
Yanhui Cai, Yizhu Chen, Yiqiu Xia, Siyang Zheng, Zhiwen Liu, Kebin Shi
{"title":"Single-Lens Light-Sheet Fluorescence Microscopy Based on Micro-Mirror Array","authors":"Yanhui Cai,&nbsp;Yizhu Chen,&nbsp;Yiqiu Xia,&nbsp;Siyang Zheng,&nbsp;Zhiwen Liu,&nbsp;Kebin Shi","doi":"10.1002/lpor.202100026","DOIUrl":null,"url":null,"abstract":"<p>Conventional light sheet fluorescence microscopy (LSFM) utilizes two perpendicularly arranged objective lenses for optical excitation and detection, respectively. Such a configuration often limits the use of high-numerical-aperture (NA) objectives or requires specially designed long-working-distance objectives. Here, a LSFM based on a micro-mirror array (MMA) to enable light sheet imaging with a single objective lens is reported. The planar fluorescent emission excited by the light sheet illumination is collected by the same objective, relayed onto an MMA and detected by a side-view camera. The proposed scheme makes LSFM compatible to single objective imaging system and shows promising candidacy for high spatiotemporal imaging.</p>","PeriodicalId":204,"journal":{"name":"Laser & Photonics Reviews","volume":"16 8","pages":""},"PeriodicalIF":10.0000,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lpor.202100026","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Laser & Photonics Reviews","FirstCategoryId":"101","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/lpor.202100026","RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"OPTICS","Score":null,"Total":0}
引用次数: 4

Abstract

Conventional light sheet fluorescence microscopy (LSFM) utilizes two perpendicularly arranged objective lenses for optical excitation and detection, respectively. Such a configuration often limits the use of high-numerical-aperture (NA) objectives or requires specially designed long-working-distance objectives. Here, a LSFM based on a micro-mirror array (MMA) to enable light sheet imaging with a single objective lens is reported. The planar fluorescent emission excited by the light sheet illumination is collected by the same objective, relayed onto an MMA and detected by a side-view camera. The proposed scheme makes LSFM compatible to single objective imaging system and shows promising candidacy for high spatiotemporal imaging.

Abstract Image

基于微镜阵列的单透镜光片荧光显微镜
传统的光片荧光显微镜(LSFM)利用两个垂直排列的物镜分别进行光激发和检测。这种结构通常限制了高数值孔径物镜的使用,或者需要专门设计的长工作距离物镜。本文报道了一种基于微镜阵列(MMA)的单物镜光片成像的LSFM。由光片照射激发的平面荧光发射由同一物镜收集,传递到MMA上,由侧视相机检测。该方案兼容单物镜成像系统,在高时空成像领域具有广阔的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
14.20
自引率
5.50%
发文量
314
审稿时长
2 months
期刊介绍: Laser & Photonics Reviews is a reputable journal that publishes high-quality Reviews, original Research Articles, and Perspectives in the field of photonics and optics. It covers both theoretical and experimental aspects, including recent groundbreaking research, specific advancements, and innovative applications. As evidence of its impact and recognition, Laser & Photonics Reviews boasts a remarkable 2022 Impact Factor of 11.0, according to the Journal Citation Reports from Clarivate Analytics (2023). Moreover, it holds impressive rankings in the InCites Journal Citation Reports: in 2021, it was ranked 6th out of 101 in the field of Optics, 15th out of 161 in Applied Physics, and 12th out of 69 in Condensed Matter Physics. The journal uses the ISSN numbers 1863-8880 for print and 1863-8899 for online publications.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信