Mapping QTLs for Male Sterile Gene in YM-Type Thermo-Sensitive Male Sterile Line of Wheat

Q3 Agricultural and Biological Sciences
Ju-Hong ZHOU , Ke LI , Bei-Ru HE , Yin-Gang HU
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引用次数: 1

Abstract

The sterile gene of YM-type thermo-sensitive male sterile wheat (Triticum aestivum L.) line has been mapped on chromosome arm 1BS, which was more than 10 cM distant from the adjacent molecular markers. For detecting markers with short distances to this gene, a population with 200 F2 plants was constructed from the cross between ATM3314 and the restorer line Chinese Spring. Among 20 simple sequence repeat (SSR) markers distributing evenly on 1BS, 5 SSRs showed polymorphism between the parents and between the male sterile and fertile bulks. A 1BS partial linkage map carrying the 5 markers was obtained and QTLs for the male sterility were identified using composite interval mapping method. One major QTL and one minor QTL were detected and designated rfv1-1 and rfv1-2, respectively. QTL rfv1-1 (LOD = 8.80) was located between markers Xgwm18 and Xwmc406 with the genetic distances of 6.0 cM and 4.6 cM to the adjacent markers, respectively. On this locus, the additive and dominant effects were 23.87 and 10.44, respectively, which accounted for 23.9% of the phenotypic variation. QTL rfv1-2 (LOD = 3.10) was mapped between markers Xwmc406 and Xbarc8 with the genetic distances of 4.0 cM and 3.4 cM, respectively. This locus had additive effect of 17.59 and dominant effect of 5.99, and explained 7.8% of the phenotypic variation. These results are propitious for fine mapping and positional cloning of this male sterile gene.

小麦m型温敏雄性不育系雄性不育基因qtl定位
在距离相邻分子标记10 cM以上的染色体臂1BS上,成功定位了m型温敏雄性不育小麦(Triticum aestivum L.)的不育基因。为了检测该基因的近距离标记,以ATM3314与恢复系中华春为材料,构建了200株F2的群体。在均匀分布于1BS上的20个SSR标记中,有5个SSR在亲本之间、雄性不育块与可育块之间存在多态性。获得了5个标记的1BS部分连锁图谱,并用复合区间作图法鉴定了雄性不育的qtl。检测到1个主要QTL和1个次要QTL,分别命名为rfv1-1和rfv1-2。QTL rfv1-1 (LOD = 8.80)位于Xgwm18和Xwmc406之间,与相邻标记的遗传距离分别为6.0 cM和4.6 cM。在该位点上加性效应和显性效应分别为23.87和10.44,占表型变异的23.9%。QTL rfv1-2 (LOD = 3.10)在标记Xwmc406和Xbarc8之间定位,遗传距离分别为4.0 cM和3.4 cM。加性效应为17.59,显性效应为5.99,解释了7.8%的表型变异。这些结果为该雄性不育基因的精细定位和定位克隆提供了有利条件。
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来源期刊
CiteScore
1.50
自引率
0.00%
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0
审稿时长
30 weeks
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