Construction of Molecular Linkage Maps Using SRAP Markers in Sweetpotato

Q3 Agricultural and Biological Sciences

Acta Agronomica Sinica Pub Date : 2010-07-01 DOI:10.1016/S1875-2780(09)60065-1

Ai-Xian LI , Qing-Chang LIU , Qing-Mei WANG , Li-Ming ZHANG , Hong ZHAI , Shu-Zhen LIU

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引用次数: 20

Abstract

The objective of this study was to construct a linkage map of sweetpotato [Ipomoea batatas (L.) Lam.] based on Chinese cultivars with higher marker density than previous maps. Cultivars Luoxushu 8 (female parent) and Zhengshu 20 (male parent) with obvious differences in starch content, yield, β-carotene content, and disease resistance were crossed to produce a segregation population with 240 F₁ individuals. Among the total 800 sequence-related amplified polymorphism (SRAP) markers, 770 and 523 markers showed polymorphism in the female and male parents, respectively. Using the “double pseudo testcross” strategy, 2 linkage maps were constructed by means of JoinMap 3.0. The linkage maps were composed of 473 markers in 81 linkage groups for Luoxushu 8 and 328 markers in 66 linkage groups for Zhengshu 20. The former map covered 5802.46 cM with an average marker interval of 10.16 cM, and latter one covered 3967.90 cM with an average of 12.02 cM. Both linkage maps may facilitate marker-assisted selection, gene localization, and gene cloning in sweetpotato.

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利用SRAP标记构建甘薯分子连锁图谱

本研究的目的是构建甘薯[Ipomoea batatas (L.)]的连锁图谱。林。]，标记密度比以前的地图更高。以淀粉含量、产量、β-胡萝卜素含量和抗病性均有显著差异的品种罗树8号(母本)和郑树20号(父本)杂交，形成一个有240个F1个体的分离群体。在800个序列相关扩增多态性(SRAP)标记中，分别有770个和523个标记在母本和父本中存在多态性。采用“双伪测试交叉”策略，利用JoinMap 3.0构建了2个联动图。该连锁图谱由罗树8号的81个连锁群中的473个标记和正树20号的66个连锁群中的328个标记组成。前者地图覆盖5802.46 cM，平均标记间隔10.16 cM;后者地图覆盖3967.90 cM，平均标记间隔12.02 cM。这两种连锁图谱可以促进甘薯的标记辅助选择、基因定位和基因克隆。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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