Cloning and Characterization of WOX4 Gene from Vitis vinifera L. Involved in Stem Cell Regulation

Agricultural Sciences in China Pub Date : 2011-12-01 DOI:10.1016/S1671-2927(11)60186-7

Ru DAI , Hai-peng JIN , Zeng WANG , Perl Avihai , Hai-ying XU , Wen ZHANG , Shang-wu CHEN , Hui-qin MA

{"title":"Cloning and Characterization of WOX4 Gene from Vitis vinifera L. Involved in Stem Cell Regulation","authors":"Ru DAI , Hai-peng JIN , Zeng WANG , Perl Avihai , Hai-ying XU , Wen ZHANG , Shang-wu CHEN , Hui-qin MA","doi":"10.1016/S1671-2927(11)60186-7","DOIUrl":null,"url":null,"abstract":"<div><h3>Abstract</h3>Wuschel-related homeobox (WOX) genes play essential, specific, and sometimes redundant roles in plant embryo development, shoot and root meristem maintenance, and plant development. Though much information was quickly gained with members of the WOX gene family of Arabidopsis, monocotyledonous crops, and gymnospermous conifers, little is known about perennial woody plants. In this study, we isolated the first WOX gene family member from grape (Vitis vinifera L. cv. Cabernet Sauvignon), and named it VvWOX4 based on its characteristic domains and phylogenetic analysis. The identity of VvWOX4 was validated by MALDI-TOF MS and Western blot with polyclonal antibody against Arabidopsis thaliana Wuschel. Functional analysis showed that VvWOX4 markedly increased shoot primordia structures when overexpressed under CaMV 35S promoter in tobacco. A different expression pattern was found for VvVOX4 compared with AtWUCHEL and its expression was detected in unique organs of grapevines. Besides the expression in the vegetative shoot apical meristem (SAM) of grape shoot tips, VvWOX4 is expressed in dormant winter buds, inflorescence, young leaves, and tendril tips, but not in root tips. In young leaves, the expression of VvWOX4 is strongly upregulated by wounding, and also by plant growth regulators such as 2 mg L−1 2,4-D, 1 mg L−1 NAA and 1 mg L−1 BAP treatments, while downregulation was monitored by 1 mg L−1 IBA treatment, and there was no response to 0.5 mg L−1 GA3 treatment. Together, our results revealed the first member of grape WOX gene family and indicated different roles and regulation of VvWOX4 in the perennial woody crop grapevine.</div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 12","pages":"Pages 1861-1871"},"PeriodicalIF":0.0000,"publicationDate":"2011-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60186-7","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agricultural Sciences in China","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1671292711601867","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 5

Abstract

Wuschel-related homeobox (WOX) genes play essential, specific, and sometimes redundant roles in plant embryo development, shoot and root meristem maintenance, and plant development. Though much information was quickly gained with members of the WOX gene family of Arabidopsis, monocotyledonous crops, and gymnospermous conifers, little is known about perennial woody plants. In this study, we isolated the first WOX gene family member from grape (Vitis vinifera L. cv. Cabernet Sauvignon), and named it VvWOX4 based on its characteristic domains and phylogenetic analysis. The identity of VvWOX4 was validated by MALDI-TOF MS and Western blot with polyclonal antibody against Arabidopsis thaliana Wuschel. Functional analysis showed that VvWOX4 markedly increased shoot primordia structures when overexpressed under CaMV 35S promoter in tobacco. A different expression pattern was found for VvVOX4 compared with AtWUCHEL and its expression was detected in unique organs of grapevines. Besides the expression in the vegetative shoot apical meristem (SAM) of grape shoot tips, VvWOX4 is expressed in dormant winter buds, inflorescence, young leaves, and tendril tips, but not in root tips. In young leaves, the expression of VvWOX4 is strongly upregulated by wounding, and also by plant growth regulators such as 2 mg L⁻¹ 2,4-D, 1 mg L⁻¹ NAA and 1 mg L⁻¹ BAP treatments, while downregulation was monitored by 1 mg L⁻¹ IBA treatment, and there was no response to 0.5 mg L⁻¹ GA3 treatment. Together, our results revealed the first member of grape WOX gene family and indicated different roles and regulation of VvWOX4 in the perennial woody crop grapevine.

查看原文本刊更多论文

葡萄干细胞调控WOX4基因的克隆与鉴定

摘要/ abstract摘要:WOX基因在植物胚发育、茎根分生组织维持和植物发育过程中发挥着重要的、特异的、有时甚至是多余的作用。虽然对拟南芥、单子叶作物和裸子针叶树的WOX基因家族成员的了解很快就得到了很多，但对多年生木本植物的了解却很少。在本研究中，我们从葡萄(Vitis vinifera L. cv.)中分离到了首个WOX基因家族成员。根据赤霞珠(Cabernet Sauvignon)的特征域和系统发育分析，将其命名为VvWOX4。利用MALDI-TOF MS和Western blot对拟南芥(Arabidopsis thaliana Wuschel)多克隆抗体进行鉴定。功能分析表明，在CaMV 35S启动子下过表达的VvWOX4显著增加了烟草茎原基结构。与AtWUCHEL相比，VvVOX4的表达模式不同，并且在葡萄的特定器官中检测到其表达。除了在葡萄芽尖的营养芽顶分生组织(SAM)中表达外，VvWOX4在休眠的冬芽、花序、幼叶和卷须尖中表达，而在根尖中不表达。在幼叶中，损伤和植物生长调节剂(2mg L−1 2,4- d、1mg L−1 NAA和1mg L−1 BAP)处理能显著上调VvWOX4的表达，而1mg L−1 IBA处理能下调表达，而0.5 mg L−1 GA3处理则无反应。研究结果揭示了葡萄WOX基因家族的首个成员，并揭示了该基因在多年生木本作物葡萄中的不同作用和调控机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Agricultural Sciences in China AGRICULTURE, MULTIDISCIPLINARY-

自引率

0.00%

发文量

审稿时长

3.2 months