The Potential Role of Circulating Long Miscellaneous RNAs in the Diagnosis and Prognosis of Hepatitis C Related Hepatocellular Carcinoma.

IF 3.6 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Non-Coding RNA Pub Date : 2023-10-11 DOI:10.3390/ncrna9050062

Shimaa Abdelsattar, Sally A Fahim, Hala F M Kamel, Hiba Al-Amodi, Zeinab A Kasemy, Fatma O Khalil, Mahmoud S Abdallah, Hanan M Bedair, Abdel-Naser Abdel-Atty Gadallah, Aliaa Sabry, Mohamed A Sakr, Mahmoud Selim, Eman M Abd El Gayed

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引用次数: 0

Abstract

Ribonucleic acids (RNAs) are important regulators of gene expression and crucial for the progression of hepatocellular carcinoma (HCC). This study was designed to determine the diagnostic and prognostic utility of the circulating long miscellaneous RNAs; LINC01419, AK021443, and AF070632 in HCV-related HCC patients. Real-time PCR was used to measure their relative expression levels in the plasma of 194 HCV patients, 120 HCV-related HCC patients and 120 healthy controls. LINC01419 and AK021443 expression levels had significantly increasing linear trend estimates while AF070632 was dramatically downregulated in HCC compared to HCV. Interestingly, LINC01419 and AK021443 served as more significant diagnostic biomarkers for HCC than AF070632 and AFP. Multivariate analysis with cox regression revealed that the high expression of AK021443 [HR = 10.06, CI95%: 3.36-30.07], the high expression of LINC01419 [HR 4.13, CI95%: 1.32-12.86], and the low expression of AF070632 [HR = 2.70, CI95%: 1.07-6.81] were significant potential prognostic factors for HCC. Besides, the Kaplan-Meier analysis showed that HCC patients with high LIN01419 and AK021443 and low AF070632 expression levels had shorter OS. The circulating LINC01419 and AK021443 can be used as noninvasive potential biomarkers for diagnosis and prognosis of HCV-related HCC patients than AF070632 providing new targets for limiting the progression of the disease.

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循环长杂RNA在丙型肝炎相关肝细胞癌诊断和预后中的潜在作用。

核糖核酸（RNA）是基因表达的重要调节因子，对肝细胞癌（HCC）的进展至关重要。本研究旨在确定循环长杂RNA的诊断和预后效用；LINC01419、AK021443和AF070632在HCV相关HCC患者中的应用。采用实时聚合酶链式反应测定194名HCV患者、120名HCV相关HCC患者和120名健康对照者血浆中它们的相对表达水平。LINC01419和AK021443的表达水平具有显著增加的线性趋势估计，而与HCV相比，AF070632在HCC中显著下调。有趣的是，LINC01419和AK021443是比AF070632和AFP更重要的HCC诊断生物标志物。采用cox回归的多因素分析显示，AK021443的高表达[HR=10.06，CI95%:3.36-30.07]、LINC01419的高表达[HR4.13，CI95%1.32-12.86]和AF070632的低表达[HR=2.70，CI95%21.07-6.81]是HCC的重要潜在预后因素。此外，Kaplan-Meier分析显示，具有高LIN01419和AK021443以及低AF070632表达水平的HCC患者具有较短的OS。与AF070632相比，循环的LINC01419和AK021443可作为诊断和预后HCV相关HCC患者的非侵入性潜在生物标志物，为限制疾病进展提供了新的靶点。

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来源期刊

Non-Coding RNA Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

6.70

自引率

4.70%

发文量

审稿时长

10 weeks

期刊介绍： Functional studies dealing with identification, structure-function relationships or biological activity of: small regulatory RNAs (miRNAs, siRNAs and piRNAs) associated with the RNA interference pathway small nuclear RNAs, small nucleolar and tRNAs derived small RNAs other types of small RNAs, such as those associated with splice junctions and transcription start sites long non-coding RNAs, including antisense RNAs, long ''intergenic'' RNAs, intronic RNAs and ''enhancer'' RNAs other classes of RNAs such as vault RNAs, scaRNAs, circular RNAs, 7SL RNAs, telomeric and centromeric RNAs regulatory functions of mRNAs and UTR-derived RNAs catalytic and allosteric (riboswitch) RNAs viral, transposon and repeat-derived RNAs bacterial regulatory RNAs, including CRISPR RNAS Analysis of RNA processing, RNA binding proteins, RNA signaling and RNA interaction pathways: DICER AGO, PIWI and PIWI-like proteins other classes of RNA binding and RNA transport proteins RNA interactions with chromatin-modifying complexes RNA interactions with DNA and other RNAs the role of RNA in the formation and function of specialized subnuclear organelles and other aspects of cell biology intercellular and intergenerational RNA signaling RNA processing structure-function relationships in RNA complexes RNA analyses, informatics, tools and technologies: transcriptomic analyses and technologies development of tools and technologies for RNA biology and therapeutics Translational studies involving long and short non-coding RNAs: identification of biomarkers development of new therapies involving microRNAs and other ncRNAs clinical studies involving microRNAs and other ncRNAs.