Pseudomonas aeruginosa biofilm exopolysaccharides: assembly, function, and degradation.

IF 10.1 2区 生物学 Q1 MICROBIOLOGY
Andreea A Gheorghita, Daniel J Wozniak, Matthew R Parsek, P Lynne Howell
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引用次数: 0

Abstract

The biofilm matrix is a fortress; sheltering bacteria in a protective and nourishing barrier that allows for growth and adaptation to various surroundings. A variety of different components are found within the matrix including water, lipids, proteins, extracellular DNA, RNA, membrane vesicles, phages, and exopolysaccharides. As part of its biofilm matrix, Pseudomonas aeruginosa is genetically capable of producing three chemically distinct exopolysaccharides - alginate, Pel, and Psl - each of which has a distinct role in biofilm formation and immune evasion during infection. The polymers are produced by highly conserved mechanisms of secretion, involving many proteins that span both the inner and outer bacterial membranes. Experimentally determined structures, predictive modelling of proteins whose structures are yet to be solved, and structural homology comparisons give us insight into the molecular mechanisms of these secretion systems, from polymer synthesis to modification and export. Here, we review recent advances that enhance our understanding of P. aeruginosa multiprotein exopolysaccharide biosynthetic complexes, and how the glycoside hydrolases/lyases within these systems have been commandeered for antimicrobial applications.

铜绿假单胞菌生物膜胞外多糖:组装、功能和降解。
生物膜基质是一座堡垒;将细菌遮蔽在一个保护性和滋养性屏障中,使其能够生长和适应各种环境。在基质中发现了多种不同的成分,包括水、脂质、蛋白质、细胞外DNA、RNA、膜囊泡、噬菌体和胞外多糖。作为其生物膜基质的一部分,铜绿假单胞菌在基因上能够产生三种化学上不同的胞外多糖——藻酸盐、Pel和Psl——每种都在感染期间的生物膜形成和免疫逃避中发挥着不同的作用。这些聚合物是由高度保守的分泌机制产生的,涉及许多横跨细菌内膜和外膜的蛋白质。实验确定的结构、结构有待解决的蛋白质的预测建模以及结构同源性比较使我们深入了解了这些分泌系统的分子机制,从聚合物合成到修饰和输出。在这里,我们回顾了最近的进展,这些进展增强了我们对铜绿假单胞菌多蛋白胞外多糖生物合成复合物的理解,以及这些系统中的糖苷水解酶/裂解酶是如何被用于抗菌应用的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
FEMS microbiology reviews
FEMS microbiology reviews 生物-微生物学
CiteScore
17.50
自引率
0.90%
发文量
45
审稿时长
6-12 weeks
期刊介绍: Title: FEMS Microbiology Reviews Journal Focus: Publishes reviews covering all aspects of microbiology not recently surveyed Reviews topics of current interest Provides comprehensive, critical, and authoritative coverage Offers new perspectives and critical, detailed discussions of significant trends May contain speculative and selective elements Aimed at both specialists and general readers Reviews should be framed within the context of general microbiology and biology Submission Criteria: Manuscripts should not be unevaluated compilations of literature Lectures delivered at symposia must review the related field to be acceptable
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