A Novel Peptide COX_52-69 Inhibits High Glucose-induced Insulin Secretion by Modulating BK Channel Activity.

IF 1.9 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current protein & peptide science Pub Date : 2024-01-01 DOI:10.2174/0113892037249620231010063637

Qian Lin, Jingtao Liu, Hengling Chen, Wenwu Hu, Weiqiong Lei, Meijie Wang, Xianguang Lin, Yongning Zhang, Huiting Ai, Su Chen, Chenhong Li

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Abstract

Background: Excessive insulin is the leading cause of metabolic syndromes besides hyperinsulinemia. Insulin-lowering therapeutic peptides have been poorly studied and warrant urgent attention.

Objectives: The main purpose of this study, was to introduce a novel peptide COX_52-69 that was initially isolated from the porcine small intestine and possessed the ability to inhibit insulin secretion under high-glucose conditions by modulating large conductance Ca²⁺-activated K⁺ channels (BK channels) activity.

Methods and results: Enzyme-linked immunosorbent assay results indicate that COX_52-69 supressed insulin release induced by high glucose levels in pancreatic islets and animal models. Furthermore, electrophysiological data demonstrated that COX_52-69 can increase BK channel currents and hyperpolarize cell membranes. Thus, cell excitability decreased, corresponding to a reduction in insulin secretion.

Conclusion: Our study provides a novel approach to modulate high glucose-stimulated insulin secretion in patients with hyperinsulinemia.

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一种新型肽COX52-69通过调节BK通道活性抑制高糖诱导的胰岛素分泌。

背景：除了高胰岛素血症外，过量的胰岛素是代谢综合征的主要原因。胰岛素降低治疗肽的研究很少，急需引起重视。目的：本研究的主要目的是介绍一种新的肽COX52-69，该肽最初从猪小肠中分离，具有在高糖条件下通过调节大电导Ca2+激活的K+通道（BK通道）活性来抑制胰岛素分泌的能力。方法和结果：酶联免疫吸附试验结果表明，COX52-69抑制高糖诱导的胰岛和动物模型中的胰岛素释放。此外，电生理学数据表明COX52-69可以增加BK通道电流并使细胞膜超极化。因此，细胞兴奋性降低，对应于胰岛素分泌的减少。结论：我们的研究为调节高胰岛素血症患者高糖刺激的胰岛素分泌提供了一种新的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current protein & peptide science 生物-生化与分子生物学

CiteScore

5.20

自引率

0.00%

发文量

审稿时长

6 months

期刊介绍： Current Protein & Peptide Science publishes full-length/mini review articles on specific aspects involving proteins, peptides, and interactions between the enzymes, the binding interactions of hormones and their receptors; the properties of transcription factors and other molecules that regulate gene expression; the reactions leading to the immune response; the process of signal transduction; the structure and function of proteins involved in the cytoskeleton and molecular motors; the properties of membrane channels and transporters; and the generation and storage of metabolic energy. In addition, reviews of experimental studies of protein folding and design are given special emphasis. Manuscripts submitted to Current Protein and Peptide Science should cover a field by discussing research from the leading laboratories in a field and should pose questions for future studies. Original papers, research articles and letter articles/short communications are not considered for publication in Current Protein & Peptide Science.