Anesthesia and surgery induce changes in endogenous brain protective protein (RNF146) and delirium-like behavior in aged rats.

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Acta biochimica Polonica Pub Date : 2023-10-26 DOI:10.18388/abp.2020_6720

Yubo Gao, Xu Han, Xiuhua Li, Shaling Tang, Chun Zhang, Xiaoxia Yang, Majid Alhomrani, Abdulhakeem S Alamri, Ghulam Nabi, Xinli Ni

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引用次数: 0

Abstract

Background: Postoperative delirium (POD) is a common complication after anesthesia and surgery, especially in the elderly. RNF146 has neuroprotective effects in cerebral ischemia, hypoxia, and chronic neurological diseases. However, whether RNF146 expression is related to the occurrence and development of POD remains unclear. Therefore, in this study, we aimed to determine whether RNF146 is involved in the occurrence of POD.

Methods: (Sprague-Dawley) male rats (18 months old) were splenectomized under sevoflurane anesthesia. The cognitive function of rats at 1, 3, and 7 d after anesthesia and surgery was evaluated. Changes in the expression of neuroinflammatory cytokines, IL-6 and IL-10, and RNF146 were measured in the hippocampus in both control group (con) and anesthesia (AS) group. We examined cognitive outcomes and expression of inflammatory factors and RNF146 in con and AS mice using cluster analysis.

Results: The cognitive ability and mobility of rats after anesthesia and surgery at day 1, 3, and 7 decreased, especially at day 3. Similarly, the expression of neuroinflammatory factors and RNF146 increased after anesthesia and surgery at day 1, 3, and 7, and the increase was highest at day 3. The clustering and correlation analysis of RNF146 expression in the hippocampi of elderly rats revealed a correlation between POD and neuroinflammation resulting from anesthesia and surgery.

Conclusion: Anesthesia and surgery can lead to POD and neuroinflammation. The expression of RNF146 correlates with delirium and neuroinflammation caused by anesthesia and surgery.

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麻醉和手术诱导老年大鼠内源性脑保护蛋白（RNF146）和谵妄样行为的变化。

背景：术后谵妄（POD）是麻醉和手术后常见的并发症，尤其在老年人中。RNF146对脑缺血、缺氧和慢性神经系统疾病具有神经保护作用。然而，RNF146的表达是否与POD的发生和发展有关尚不清楚。因此，本研究旨在确定RNF146是否参与POD的发生。方法：在七氟醚麻醉下对（Sprague-Dawley）雄性大鼠（18个月大）进行脾切除。评估麻醉和手术后1、3和7天大鼠的认知功能。在对照组（con）和麻醉组（AS）的海马中测量神经炎症细胞因子IL-6和IL-10以及RNF146的表达变化。我们使用聚类分析检测了con和AS小鼠的认知结果以及炎症因子和RNF146的表达。结果：麻醉和手术后第1、3和7天，大鼠的认知能力和活动能力下降，尤其是在第3天。类似地，神经炎症因子和RNF146的表达在麻醉和手术后的第1、3和7天增加，并且在第3天增加最高。RNF146在老年大鼠海马中表达的聚类和相关性分析揭示了POD与麻醉和手术引起的神经炎症之间的相关性。结论：麻醉和手术可导致POD和神经炎症。RNF146的表达与麻醉和手术引起的谵妄和神经炎症相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta biochimica Polonica 生物-生化与分子生物学

CiteScore

2.40

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Acta Biochimica Polonica is a journal covering enzymology and metabolism, membranes and bioenergetics, gene structure and expression, protein, nucleic acid and carbohydrate structure and metabolism.