Long-term Xenopus laevis tadpole -heart-organ-culture: Physiological changes in cholinergic and adrenergic sensitivities of tadpole heart with thyroxine-treatment.

IF 2.1 Q3 PHYSIOLOGY

Current research in physiology Pub Date : 2023-06-07 eCollection Date: 2023-01-01 DOI:10.1016/j.crphys.2023.100100

Hideki Hanada, Fumihiro Morishita, Seigo Sanoh, Keiko Kashiwagi, Akihiko Kashiwagi

{"title":"Long-term Xenopus laevis tadpole -heart-organ-culture: Physiological changes in cholinergic and adrenergic sensitivities of tadpole heart with thyroxine-treatment.","authors":"Hideki Hanada, Fumihiro Morishita, Seigo Sanoh, Keiko Kashiwagi, Akihiko Kashiwagi","doi":"10.1016/j.crphys.2023.100100","DOIUrl":null,"url":null,"abstract":"The present study clarified changes in physiological sensitivities of cultured Nieuwkoop and Faber stage 57 Xenopus laevis tadpole-organ-heart exposed to thyroxine (T4) using acetylcholine (ACh), norepinephrine (NE) and atropine. For preliminary life span and the chemical tests, 60% minimum essential medium (MEM), two types of modified Hank's balanced salt-solution-culture-media (MHBSS-CM) I and II containing relatively lower concentrations of amino acids and collagen were prepared. In preliminary lifespan-test of cultured tadpole hearts, the hearts maintained in 60% MEM was 50 days on average, whereas that of the tadpole-hearts in MHBSS-CMs was extended by 109 days on average, showing superior effectiveness of MHBSS-CMs. 4 min-stimulation by 5 × 10-9 M T4 tended to increase the tadpole heartbeat. 10-9 M ACh decreased the tadpole heartbeat. Frog-heart at 2-4 weeks after metamorphosis completion and tadpole heart treated with 5 × 10-10 M T4 for 45 h also responded to 10-9 M ACh, and low-resting hearts were restored to the control level with the competitive muscarinic antagonist 10-8 M atropine, whereas excessive exposure of 10-5 M atropine to T4-treated tadpole heart did not increase heartbeat in spite of the increased frog heartbeat over the control. 10-14 -10-12 M NE increase the tadpole heartbeat in a concentration-dependent manner, however, 10-12 M NE did not act to stimulate adrenergic receptors on both T4-treated tadpole- and the frog-hearts. These results suggest that T4 induces the desensitization of atropine-sensitive muscarinic and adrenergic receptors in organ-cultured tadpole-heart.","PeriodicalId":72753,"journal":{"name":"Current research in physiology","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2023-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10724204/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current research in physiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.crphys.2023.100100","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"PHYSIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The present study clarified changes in physiological sensitivities of cultured Nieuwkoop and Faber stage 57 Xenopus laevis tadpole-organ-heart exposed to thyroxine (T₄) using acetylcholine (ACh), norepinephrine (NE) and atropine. For preliminary life span and the chemical tests, 60% minimum essential medium (MEM), two types of modified Hank's balanced salt-solution-culture-media (MHBSS-CM) I and II containing relatively lower concentrations of amino acids and collagen were prepared. In preliminary lifespan-test of cultured tadpole hearts, the hearts maintained in 60% MEM was 50 days on average, whereas that of the tadpole-hearts in MHBSS-CMs was extended by 109 days on average, showing superior effectiveness of MHBSS-CMs. 4 min-stimulation by 5 × 10^-9 M T₄ tended to increase the tadpole heartbeat. 10^-9 M ACh decreased the tadpole heartbeat. Frog-heart at 2-4 weeks after metamorphosis completion and tadpole heart treated with 5 × 10^-10 M T₄ for 45 h also responded to 10^-9 M ACh, and low-resting hearts were restored to the control level with the competitive muscarinic antagonist 10^-8 M atropine, whereas excessive exposure of 10^-5 M atropine to T₄-treated tadpole heart did not increase heartbeat in spite of the increased frog heartbeat over the control. 10^-14 -10^-12 M NE increase the tadpole heartbeat in a concentration-dependent manner, however, 10^-12 M NE did not act to stimulate adrenergic receptors on both T₄-treated tadpole- and the frog-hearts. These results suggest that T₄ induces the desensitization of atropine-sensitive muscarinic and adrenergic receptors in organ-cultured tadpole-heart.

查看原文本刊更多论文

蝌蚪心脏器官的长期培养：甲状腺素处理对蝌蚪心脏胆碱能和肾上腺素能敏感性的生理变化

本研究利用乙酰胆碱（ACh）、去甲肾上腺素（NE）和阿托品阐明了培养的尼乌科普和法布尔57期蝌蚪器官心脏对甲状腺素（T4）的生理敏感性变化。为进行初步寿命和化学测试，制备了 60% 的最低限度基本培养基（MEM）、两种改良汉克平衡盐溶液培养基（MHBSS-CM）I 和 II，其中氨基酸和胶原蛋白的浓度相对较低。在初步的蝌蚪心脏寿命测试中，在60%的MEM中培养的心脏平均寿命为50天，而在MHBSS-CMs中培养的蝌蚪心脏平均寿命延长了109天，显示了MHBSS-CMs的优越性。5 × 10-9 M T4 的 4 分钟刺激有增加蝌蚪心跳的趋势。10-9 M ACh 会降低蝌蚪的心跳。变态完成后2-4周的蛙心和用5×10-10 M T4处理45 h的蝌蚪心脏也对10-9 M ACh有反应，低静息心脏用竞争性毒蕈碱拮抗剂10-8 M阿托品可恢复到对照水平，而对T4处理的蝌蚪心脏过度暴露于10-5 M阿托品不会增加心跳，尽管蛙心跳比对照有所增加。10-14 -10-12 M NE能以浓度依赖的方式增加蝌蚪的心跳，然而，10-12 M NE并不能刺激T4处理的蝌蚪和蛙心上的肾上腺素能受体。这些结果表明，T4 能诱导器官培养蝌蚪心脏中对阿托品敏感的毒蕈碱和肾上腺素能受体脱敏。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Current research in physiology Physiology

CiteScore

3.20

自引率

0.00%

发文量

审稿时长

62 days