Effects of Juglone and Curcumin Administration on Expression of FABP5 and FABP9 in MCF-7 and MDA-MB-231 Breast Cancer Cell Lines

IF 1.1 Q4 CELL BIOLOGY

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology Pub Date : 2023-03-29 DOI:10.1134/S199074782310001X

D. Soyler, E. N. Korucu, E. Menevse, A. A. Azzawri, D. E. Kaya

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引用次数: 0

Abstract—

Among natural chemopreventive agents, polyphenol curcumin and naphthoquinone juglone, which has broad-spectrum anticancer activity, are highly valued. Fatty acid-binding proteins (FABPs), which vary depending on the type of cancer, are small (14–15 kDa) proteins belonging to the lipid-binding protein superfamily. FABPs are located in many tissues and play an essential role in fatty acid metabolism, cell growth, and proliferation. It is suggested that they can be used as tumor markers. The objective of this work is to study the effects of curcumin and juglone on cell viability and to evaluate their anticancer and cytotoxic effects and changes in the FABP5 and FABP9 gene expression and protein levels in breast cancer cell lines MCF-7 and MDA-MB-231. Information on FABP5 and FABP9 gene expression in BRCA (breast invasive cancer) and normal cells was collected from the GEPIA2 and UALCAN databases. MTT analysis revealed that the IC₅₀ (concentration of half maximal inhibitory effect) in MCF-7 cells was 22.4 and 16.3 μM for curcumin and juglone, respectively, and in MDA-MB-231 cells, 10.4 and 3.4 μM for curcumin and juglone, respectively. In both cell lines, FABP5 and FABP9 gene expression and protein levels were also analyzed. We found that treatment of MCF-7 cells with curcumin and juglone reduced cell viability, expression of the FABP5 and FABP9 genes, and the levels of the FABP5 and FABP9 proteins. In the MDA-MB-231 cell line, the FABP5 and FABP9 levels were increased at low doses of curcumin and juglone and decreased at higher doses.

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核桃酮和姜黄素对MCF-7和MDA-MB-231乳腺癌细胞株FABP5和FABP9表达的影响

摘要-在天然化学防癌剂中，多酚姜黄素和萘醌因具有广谱抗癌活性而备受重视。脂肪酸结合蛋白(FABPs)是脂质结合蛋白超家族的小蛋白(14-15 kDa)，根据癌症类型而变化。FABPs存在于许多组织中，在脂肪酸代谢、细胞生长和增殖中发挥重要作用。提示它们可作为肿瘤标志物。本研究旨在研究姜黄素和核桃酚酮对乳腺癌细胞系MCF-7和MDA-MB-231细胞活力的影响，并评价其抗癌和细胞毒作用以及FABP5和FABP9基因表达和蛋白水平的变化。FABP5和FABP9基因在BRCA(乳腺浸润性癌)和正常细胞中的表达信息从GEPIA2和UALCAN数据库中收集。MTT分析显示，MCF-7细胞中姜黄素和核桃酚酮的IC50(半数最大抑制效应浓度)分别为22.4和16.3 μM, MDA-MB-231细胞中姜黄素和核桃酚酮的IC50分别为10.4和3.4 μM。在两种细胞系中分析FABP5和FABP9基因表达和蛋白水平。我们发现用姜黄素和核桃酚酮处理MCF-7细胞降低了细胞活力、FABP5和FABP9基因的表达以及FABP5和FABP9蛋白的水平。在MDA-MB-231细胞系中，FABP5和FABP9水平在低剂量姜黄素和木酚酮下升高，在高剂量下降低。

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来源期刊

Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology CELL BIOLOGY-

CiteScore

1.40

自引率

0.00%

发文量

期刊介绍： Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology is an international peer reviewed journal that publishes original articles on physical, chemical, and molecular mechanisms that underlie basic properties of biological membranes and mediate membrane-related cellular functions. The primary topics of the journal are membrane structure, mechanisms of membrane transport, bioenergetics and photobiology, intracellular signaling as well as membrane aspects of cell biology, immunology, and medicine. The journal is multidisciplinary and gives preference to those articles that employ a variety of experimental approaches, basically in biophysics but also in biochemistry, cytology, and molecular biology. The journal publishes articles that strive for unveiling membrane and cellular functions through innovative theoretical models and computer simulations.