Transcriptome-wide N6-methyladenosine (m6A) methylation in soybean under Meloidogyne incognita infection

IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Xue Han, Qianqian Shi, Ziyi He, Wenwen Song, Qingshan Chen, Zhaoming Qi
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引用次数: 2

Abstract

N6-methyladenosine (m6A) is a reversible epigenetic modification of mRNA and other RNAs that plays a significant role in regulating gene expression and biological processes. However, m6A abundance, dynamics, and transcriptional regulatory mechanisms remain unexplored in the context of soybean resistance to Meloidogyne incognita. In this study, we performed a comparative analysis of transcriptome-wide m6A and metabolome profiles of soybean root tissues with and without M. incognita infection. Global m6A hypermethylation was widely induced in response to M. incognita infection and was enriched around the 3′ end of coding sequences and in 3′ UTR regions. There were 2069 significantly modified m6A sites, 594 differentially expressed genes, and 103 differentially accumulated metabolites between infected and uninfected roots, including coumestrol, psoralidin, and 2-hydroxyethylphosphonate. Among 101 m6A-modified DEGs, 34 genes were hypomethylated and upregulated, and 39 genes were hypermethylated and downregulated, indicating a highly negative correlation between m6A methylation and gene transcript abundance. A number of these m6A-modified DEGs, including WRKY70, ERF60, POD47 and LRR receptor-like serine/threonine-protein kinases, were involved in plant defense responses. Our study provides new insights into the critical role of m6A modification in early soybean responses to M. incognita.

Abstract Image

隐性根结线虫感染下大豆全转录组N6-甲基腺苷(m6A)甲基化
N6-甲基腺苷(m6A)是mRNA和其他RNA的可逆表观遗传学修饰,在调节基因表达和生物过程中发挥重要作用。然而,在大豆对南方根结线虫抗性的背景下,m6A的丰度、动力学和转录调控机制仍未得到探索。在这项研究中,我们对有和没有隐球菌感染的大豆根组织的转录组范围的m6A和代谢组图谱进行了比较分析。全局m6A超甲基化在对M.incognita感染的反应中被广泛诱导,并在编码序列的3′端和3′UTR区域富集。感染根和未感染根之间有2069个显著修饰的m6A位点、594个差异表达基因和103个差异积累的代谢产物,包括香豆素、补骨脂素和2-羟乙基膦酸酯。在101个m6A修饰的DEG中,34个基因低甲基化和上调,39个基因高甲基化和下调,表明m6A甲基化与基因转录物丰度之间存在高度负相关。许多m6A修饰的DEG,包括WRKY70、ERF60、POD47和LRR受体样丝氨酸/苏氨酸蛋白激酶,参与了植物防御反应。我们的研究为m6A修饰在大豆早期对隐翅虫反应中的关键作用提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.70
自引率
2.80%
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