The application of CRISPR/Cas technologies to Brassica crops: current progress and future perspectives

IF 4.6 4区农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

aBIOTECH Pub Date : 2022-07-02 DOI:10.1007/s42994-022-00076-3

Jun Li, Xiaoxiao Yu, Chao Zhang, Na Li, Jianjun Zhao

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引用次数: 8

Abstract

Brassica species are a global source of nutrients and edible vegetable oil for humans. However, all commercially important Brassica crops underwent a whole-genome triplication event, hindering the development of functional genomics and breeding programs. Fortunately, clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) technologies, by allowing multiplex and precise genome engineering, have become valuable genome-editing tools and opened up new avenues for biotechnology. Here, we review current progress in the use of CRISPR/Cas technologies with an emphasis on the latest breakthroughs in precise genome editing. We also summarize the application of CRISPR/Cas technologies to Brassica crops for trait improvements. Finally, we discuss the challenges and future directions of these technologies for comprehensive application in Brassica crops. Ongoing advancement in CRISPR/Cas technologies, in combination with other achievements, will play a significant role in the genetic improvement and molecular breeding of Brassica crops.

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CRISPR/Cas技术在芸苔属作物中的应用现状与展望

芸苔属植物是全球营养物质和人类食用植物油的来源。然而，所有商业上重要的芸苔属作物都经历了全基因组三倍化事件，阻碍了功能基因组学和育种计划的发展。幸运的是，集群规则间隔短回文重复序列（CRISPR）/CRISPR相关（Cas）技术通过允许多重和精确的基因组工程，已成为有价值的基因组编辑工具，并为生物技术开辟了新的途径。在这里，我们回顾了CRISPR/Cas技术的使用进展，重点介绍了精确基因组编辑的最新突破。我们还总结了CRISPR/Cas技术在芸苔属作物性状改良中的应用。最后，我们讨论了这些技术在芸苔属作物中综合应用的挑战和未来方向。CRISPR/Cas技术的不断进步，加上其他成就，将在芸苔属作物的遗传改良和分子育种中发挥重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

aBIOTECH

CiteScore

7.70

自引率

2.80%

发文量