NMR chemical shift assignments of RNA oligonucleotides to expand the RNA chemical shift database

IF 0.8 4区生物学 Q4 BIOPHYSICS

Biomolecular NMR Assignments Pub Date : 2021-08-27 DOI:10.1007/s12104-021-10049-0

Yaping Liu, Anita Kotar, Tracy L. Hodges, Kyrillos Abdallah, Mallak H. Taleb, Brayden A. Bitterman, Sara Jaime, Kyle J. Schaubroeck, Ethan Mathew, Nicholas W. Morgenstern, Anthony Lohmeier, Jordan L. Page, Matt Ratanapanichkich, Grace Arhin, Breanna L. Johnson, Stanislav Cherepanov, Stephen C. Moss, Gisselle Zuniga, Nicholas J. Tilson, Zoe C. Yeoh, Bruce A. Johnson, Sarah C. Keane

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引用次数: 4

Abstract

RNAs play myriad functional and regulatory roles in the cell. Despite their significance, three-dimensional structure elucidation of RNA molecules lags significantly behind that of proteins. NMR-based studies are often rate-limited by the assignment of chemical shifts. Automation of the chemical shift assignment process can greatly facilitate structural studies, however, accurate chemical shift predictions rely on a robust and complete chemical shift database for training. We searched the Biological Magnetic Resonance Data Bank (BMRB) to identify sequences that had no (or limited) chemical shift information. Here, we report the chemical shift assignments for 12 RNA hairpins designed specifically to help populate the BMRB.

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RNA寡核苷酸的核磁共振化学移位分配扩展RNA化学移位数据库

rna在细胞中发挥着无数的功能和调节作用。尽管RNA分子具有重要意义，但其三维结构的阐明却远远落后于蛋白质。基于核磁共振的研究常常受到化学位移分配的速率限制。化学位移分配过程的自动化可以极大地促进结构研究，然而，准确的化学位移预测依赖于一个强大的和完整的化学位移数据库的训练。我们检索了生物磁共振数据库(BMRB)，以确定没有(或有限)化学位移信息的序列。在这里，我们报告了12个专门设计用于帮助填充BMRB的RNA发夹的化学位移分配。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.