Enzyme activity, DNA degradation and drying times of semen, saliva and vaginal material

IF 1.9 4区医学 Q2 MEDICINE, LEGAL

Science & Justice Pub Date : 2023-09-05 DOI:10.1016/j.scijus.2023.09.001

Jonathan Finnis , Charlotte Murphy , Geraldine Davidson , Karen Alexander , Jennie Lewis , Maggie Boyce , Finlay Kennedy , David Casey , Nicola Clayson , Isla Fraser , Charlotte Hargreaves , Nighean Stevenson , Sharon Doole , Carol Rogers

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引用次数: 0

Abstract

The stability of enzyme activity and the amount of detectable DNA within liquid samples of semen, saliva and vaginal material were tested across a number of days. The concentration of DNA within neat semen and saliva samples fell significantly after one week of refrigeration. No apparent change in acid phosphatase or amylase enzyme activity was observed in neat semen and saliva samples over 96 or 72 h respectively. Changes in the enzyme activity of most of the dilute semen and saliva samples, as well as the neat vaginal material sample, were noted after 24 h. The drying times and sizes of stains produced from various volumes of neat semen, saliva and vaginal material were tested on porous and non-porous surfaces at room temperature. Larger volumes of body fluid took longer to dry and generally resulted in larger stains. Body fluids on a non-porous surface took longer to dry than on the porous surface tested.

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精液、唾液和阴道物质的酶活性、DNA降解和干燥时间

对精液、唾液和阴道材料的液体样本中酶活性的稳定性和可检测DNA的量进行了数天的测试。冷冻一周后，纯精液和唾液样本中的DNA浓度显著下降。在96或72小时内，在纯精液和唾液样本中分别没有观察到酸性磷酸酶或淀粉酶活性的明显变化。大多数稀释精液和唾液样本以及纯阴道材料样本的酶活性在24小时后发生变化。室温下，在多孔和非多孔表面上测试不同体积的纯精液、唾液和阴道材料产生的污渍的干燥时间和大小。较大体积的体液需要更长的时间才能干燥，通常会导致较大的污渍。非多孔表面上的体液干燥时间比测试的多孔表面上更长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Science & Justice 医学-病理学

CiteScore

4.20

自引率

15.80%

发文量

审稿时长

81 days

期刊介绍： Science & Justice provides a forum to promote communication and publication of original articles, reviews and correspondence on subjects that spark debates within the Forensic Science Community and the criminal justice sector. The journal provides a medium whereby all aspects of applying science to legal proceedings can be debated and progressed. Science & Justice is published six times a year, and will be of interest primarily to practising forensic scientists and their colleagues in related fields. It is chiefly concerned with the publication of formal scientific papers, in keeping with its international learned status, but will not accept any article describing experimentation on animals which does not meet strict ethical standards. Promote communication and informed debate within the Forensic Science Community and the criminal justice sector. To promote the publication of learned and original research findings from all areas of the forensic sciences and by so doing to advance the profession. To promote the publication of case based material by way of case reviews. To promote the publication of conference proceedings which are of interest to the forensic science community. To provide a medium whereby all aspects of applying science to legal proceedings can be debated and progressed. To appeal to all those with an interest in the forensic sciences.