Feeding Bacillus-based probiotics to gestating and lactating sows is an efficient method for improving immunity, gut functional status and biofilm formation by probiotic bacteria in piglets at weaning

IF 6.3

Animal Nutrition Pub Date : 2023-06-01 DOI:10.1016/j.aninu.2023.03.003

Paweł Konieczka , Karolina Ferenc , Jens N. Jørgensen , Lea H.B. Hansen , Romuald Zabielski , Jarosław Olszewski , Zdzisław Gajewski , Magdalena Mazur-Kuśnirek , Dominika Szkopek , Natalia Szyryńska , Krzysztof Lipiński

{"title":"Feeding Bacillus-based probiotics to gestating and lactating sows is an efficient method for improving immunity, gut functional status and biofilm formation by probiotic bacteria in piglets at weaning","authors":"Paweł Konieczka , Karolina Ferenc , Jens N. Jørgensen , Lea H.B. Hansen , Romuald Zabielski , Jarosław Olszewski , Zdzisław Gajewski , Magdalena Mazur-Kuśnirek , Dominika Szkopek , Natalia Szyryńska , Krzysztof Lipiński","doi":"10.1016/j.aninu.2023.03.003","DOIUrl":null,"url":null,"abstract":"<div>The effects of dietary probiotic supplementation with viable Bacillus subtilis and Bacillus amyloliquefaciens spores on sow performance, immunity, gut functional status and biofilm formation by probiotic bacteria in piglets at weaning were investigated. Ninety-six sows reared in a continuous farrowing system for one full cycle were fed gestation diets during the first 90 d of pregnancy and lactation diets until the end of lactation. The sows were fed a basal diet without probiotics (control; n = 48) or a diet supplemented with viable spores (1.1 × 109 CFU/kg of feed) (probiotic; n = 48). At 7 d of age, sucking piglets (n = 12/group) were provided prestarter creep feed until weaning at 28 d of age. The piglets in the probiotic group were supplemented with the same probiotic and dosage as their dams. Blood and colostrum collected from sows and ileal tissues collected from piglets on the day of weaning were used for analyses. Probiotics increased the weight of piglets (P = 0.077), improved the weaning weight (P = 0.039) and increased both the total creep feed consumption (P = 0.027) and litter gain (P = 0.011). Probiotics also improved the faecal score in the second (P = 0.013) week of life. The immunoglobulin G (IgG) concentrations in sow blood at farrowing and the IgM concentrations in piglet blood at weaning were higher in the probiotic group than in the control group (P = 0.046). The piglets from the probiotic-treated sows showed a higher IgM concentration in the ileal mucosa (P = 0.050) and a lower IgG concentration in the ileal mucosa (P = 0.021) compared with the piglets from control sows. The probiotic-treated piglets had a thicker ileal mucosa (P = 0.012) due to the presence of longer villi and larger Peyer's patches (P < 0.001). B. subtilis and B. amyloliquefaciens were detected in the probiotic-treated piglets but not the control piglets; these bacteria were present in the digesta and villus structures and formed structures resembling biofilms. Overall, Bacillus-based probiotic supplementation improves the health indices of sows and their piglets.</div>","PeriodicalId":62604,"journal":{"name":"Animal Nutrition","volume":"13 ","pages":"Pages 361-372"},"PeriodicalIF":6.3000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Nutrition","FirstCategoryId":"1091","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405654523000331","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 2

Abstract

The effects of dietary probiotic supplementation with viable Bacillus subtilis and Bacillus amyloliquefaciens spores on sow performance, immunity, gut functional status and biofilm formation by probiotic bacteria in piglets at weaning were investigated. Ninety-six sows reared in a continuous farrowing system for one full cycle were fed gestation diets during the first 90 d of pregnancy and lactation diets until the end of lactation. The sows were fed a basal diet without probiotics (control; n = 48) or a diet supplemented with viable spores (1.1 × 10⁹ CFU/kg of feed) (probiotic; n = 48). At 7 d of age, sucking piglets (n = 12/group) were provided prestarter creep feed until weaning at 28 d of age. The piglets in the probiotic group were supplemented with the same probiotic and dosage as their dams. Blood and colostrum collected from sows and ileal tissues collected from piglets on the day of weaning were used for analyses. Probiotics increased the weight of piglets (P = 0.077), improved the weaning weight (P = 0.039) and increased both the total creep feed consumption (P = 0.027) and litter gain (P = 0.011). Probiotics also improved the faecal score in the second (P = 0.013) week of life. The immunoglobulin G (IgG) concentrations in sow blood at farrowing and the IgM concentrations in piglet blood at weaning were higher in the probiotic group than in the control group (P = 0.046). The piglets from the probiotic-treated sows showed a higher IgM concentration in the ileal mucosa (P = 0.050) and a lower IgG concentration in the ileal mucosa (P = 0.021) compared with the piglets from control sows. The probiotic-treated piglets had a thicker ileal mucosa (P = 0.012) due to the presence of longer villi and larger Peyer's patches (P < 0.001). B. subtilis and B. amyloliquefaciens were detected in the probiotic-treated piglets but not the control piglets; these bacteria were present in the digesta and villus structures and formed structures resembling biofilms. Overall, Bacillus-based probiotic supplementation improves the health indices of sows and their piglets.

Abstract Image

查看原文本刊更多论文

给妊娠期和哺乳期母猪喂以芽孢杆菌为基础的益生菌是改善断奶仔猪免疫力、肠道功能状态和益生菌生物膜形成的有效方法

研究了日粮中添加枯草芽孢杆菌和解淀粉芽孢杆菌孢子的益生菌对断奶仔猪生产性能、免疫力、肠道功能状态和益生菌生物膜形成的影响。在一个完整周期的连续产仔系统中饲养的96头母猪在妊娠的前90天接受妊娠日粮和哺乳日粮的喂养，直到哺乳结束。母猪喂食不含益生菌的基础日粮（对照；n=48）或添加活孢子的日粮（1.1×109CFU/kg饲料）（益生菌；n=48。在7日龄时，给吮吸仔猪（n=12/组）提供动脉前爬行饲料，直到28日龄断奶。益生菌组的仔猪补充了与母猪相同的益生菌和剂量。采用断奶当天从母猪身上采集的血液和初乳，以及从仔猪身上收集的回肠组织进行分析。益生菌增加了仔猪的体重（P=0.077），改善了断奶体重（P=0.039），增加了爬行饲料的总消耗量（P=0.027）和产仔数（P=0.011）。益生菌还改善了出生后第二周的粪便评分（P=0.013）。益生菌组产仔时母猪血液中的免疫球蛋白G（IgG）浓度和断奶时仔猪血液中的IgM浓度均高于对照组（P=0.046）。与仔猪相比，益生菌处理的母猪的回肠粘膜中IgM浓度较高（P=0.050），回肠粘膜中IgG浓度较低（P=0.021）来自对照母猪。由于存在较长的绒毛和较大的Peyer’s斑块（P<；0.001），益生菌处理的仔猪的回肠粘膜较厚（P=0.012）。在益生菌处理的小猪中检测到枯草芽孢杆菌和解淀粉芽孢杆菌，但在对照小猪中未检测到；这些细菌存在于消化道和绒毛结构中，并形成类似生物膜的结构。总的来说，以芽孢杆菌为基础的益生菌补充剂可以改善母猪及其仔猪的健康指数。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Animal Nutrition Animal Science and Zoology

CiteScore

9.70

自引率

0.00%

发文量

542

审稿时长

65 days

期刊介绍： Animal Nutrition encompasses the full gamut of animal nutritional sciences and reviews including, but not limited to, fundamental aspects of animal nutrition such as nutritional requirements, metabolic studies, body composition, energetics, immunology, neuroscience, microbiology, genetics and molecular and cell biology related to primarily to the nutrition of farm animals and aquatic species. More applied aspects of animal nutrition, such as the evaluation of novel ingredients, feed additives and feed safety will also be considered but it is expected that such studies will have a strong nutritional focus. Animal Nutrition is indexed in SCIE, PubMed Central, Scopus, DOAJ, etc.