Empirical measurements of ammonium excretion in kelp forest fishes: Effects of body size, taxonomy and trophic guild

Abstract

Fishes and other consumers excrete metabolic waste products, including dissolved nutrients rich in nitrogen, which is an essential nutrient for primary production. Relatively little is known about the magnitude and variability of nutrients excreted by fishes in kelp forest ecosystems and whether consumer-derived nutrients are important for supporting kelp productivity. In this study, the supply of ammonium (NH₄⁺) excreted by the dominant fishes (30 species representing ∼85% of total fish biomass) was investigated on nearshore rocky reefs in California. Using rapid field incubations, the amount of excreted dissolved ammonium was measured as a function of body size (n = 460 individuals) and predictive models were developed relating mass to excretion rates at the family-level. Mass-specific excretion rates ranged from 0.08 to 3.45 μmol·g⁻¹·hr⁻¹, and per capita ammonium excretion rates ranged from 5.9 to 2765 μmol·individual·hr⁻¹. Ammonium excretion scaled with fish body mass to the ¾ power, as predicted by the metabolic theory of ecology; mass-specific excretion rates were higher in smaller fishes, but larger fishes contributed more ammonium per individual. When controlling for body size, ammonium excretion rates were greatest among surfperch (Embiotocidae), damselfish (Pomacentridae), and wrasses (Labridae), and the general trophic groups of planktivores and micro-carnivores. When body size differences were considered, the greatest mean excretion rates per individual were observed in larger-bodied species, such as California Sheephead (Semicossyphus pulcher) and Lingcod (Ophiodon elongatus). Empirical estimates of nutrient excretion by fishes, among the first measured in temperate kelp forests, were consistent with those in other aquatic systems. Ultimately, empirically derived excretion rates are the first step to quantifying the relative importance of consumers to nutrient cycling in kelp forest ecosystems.