Coproduction of lipids and carotenoids by the novel green alga Coelastrella sp. depending on cultivation conditions

Q1 Immunology and Microbiology

Biotechnology Reports Pub Date : 2023-03-01 DOI:10.1016/j.btre.2022.e00769

Mizuki Saito , Haruka Watanabe , Mitsuki Sasaki , Madoka Ookubo , Takashi Yarita , Masakazu Shiraiwa , Munehiko Asayama

{"title":"Coproduction of lipids and carotenoids by the novel green alga Coelastrella sp. depending on cultivation conditions","authors":"Mizuki Saito , Haruka Watanabe , Mitsuki Sasaki , Madoka Ookubo , Takashi Yarita , Masakazu Shiraiwa , Munehiko Asayama","doi":"10.1016/j.btre.2022.e00769","DOIUrl":null,"url":null,"abstract":"<div><p>A novel green alga <em>Coelastrella</em> sp. D3–1 was isolated, and its unique and significant lipid and carotenoid coproduction capability was characterised depending on cultivation conditions. The main component of produced lipids was triacylglycerol under nutrient depletion conditions, in which fatty-methyl-esters made up 20−44% of the dry cell weight (DCW) and consisted of abundant C16:0 and C18:1 fatty acids. The red (orange)-stage cells also produced a large portion of carotenoids (38.5% of the DCW) involving echinenone, canthaxanthin, and astaxanthin as major components accumulated over only 5–6 days under optimal conditions. Stress tests revealed resistance of the cells to pH 2–11, high temperatures (40–60 °C), ultraviolet irradiation, drought, and H<sub>2</sub>O<sub>2</sub> treatment, thereby showing a robust nature. Both green- and red (orange)-stage cell extracts also showed antioxidant and anti-inflammatory abilities, implying that they have significant functions as useful biorefinery materials.</p></div>","PeriodicalId":38117,"journal":{"name":"Biotechnology Reports","volume":"37 ","pages":"Article e00769"},"PeriodicalIF":0.0000,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biotechnology Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2215017X22000674","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Immunology and Microbiology","Score":null,"Total":0}

引用次数: 3

Abstract

A novel green alga Coelastrella sp. D3–1 was isolated, and its unique and significant lipid and carotenoid coproduction capability was characterised depending on cultivation conditions. The main component of produced lipids was triacylglycerol under nutrient depletion conditions, in which fatty-methyl-esters made up 20−44% of the dry cell weight (DCW) and consisted of abundant C16:0 and C18:1 fatty acids. The red (orange)-stage cells also produced a large portion of carotenoids (38.5% of the DCW) involving echinenone, canthaxanthin, and astaxanthin as major components accumulated over only 5–6 days under optimal conditions. Stress tests revealed resistance of the cells to pH 2–11, high temperatures (40–60 °C), ultraviolet irradiation, drought, and H₂O₂ treatment, thereby showing a robust nature. Both green- and red (orange)-stage cell extracts also showed antioxidant and anti-inflammatory abilities, implying that they have significant functions as useful biorefinery materials.

查看原文本刊更多论文

新型绿藻Coelastrella sp.在不同培养条件下共生产脂质和类胡萝卜素

分离到一种新的绿藻Coelastrella sp.D3-1，并根据培养条件对其独特而显著的脂质和类胡萝卜素协同生产能力进行了表征。在营养缺乏条件下，产生的脂质的主要成分是三酰甘油，其中脂肪甲酯占干细胞重量（DCW）的20−44%，由丰富的C16:0和C18:1脂肪酸组成。红（橙色）期细胞也产生了大量的类胡萝卜素（占DCW的38.5%），其中包括针叶树酮、角黄素和虾青素作为主要成分，在最佳条件下仅积累了5-6天。应激测试显示，细胞对pH 2–11、高温（40–60°C）、紫外线照射、干旱和H2O2处理具有抗性，从而表现出强健的特性。绿色和红色（橙色）阶段的细胞提取物也显示出抗氧化和抗炎能力，这意味着它们作为有用的生物炼制材料具有重要功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Biotechnology Reports Immunology and Microbiology-Applied Microbiology and Biotechnology

CiteScore

15.80

自引率

0.00%

发文量

审稿时长

55 days

期刊介绍： Biotechnology Reports covers all aspects of Biotechnology particularly those reports that are useful and informative and that will be of value to other researchers in related fields. Biotechnology Reports loves ground breaking science, but will also accept good science that can be of use to the biotechnology community. The journal maintains a high quality peer review where submissions are considered on the basis of scientific validity and technical quality. Acceptable paper types are research articles (short or full communications), methods, mini-reviews, and commentaries in the following areas: Healthcare and pharmaceutical biotechnology Agricultural and food biotechnology Environmental biotechnology Molecular biology, cell and tissue engineering and synthetic biology Industrial biotechnology, biofuels and bioenergy Nanobiotechnology Bioinformatics & systems biology New processes and products in biotechnology, bioprocess engineering.