The potency of herbal extracts and its green synthesized nanoparticle formulation as antibacterial agents against Streptococcus mutans associated biofilms

Q1 Immunology and Microbiology

Biotechnology Reports Pub Date : 2023-03-01 DOI:10.1016/j.btre.2022.e00777

Kulwadee Karnjana , Juntamanee Jewboonchu , Nattisa Niyomtham , Paveen Tangngamsakul , Kingkan Bunluepuech , Lavnaya Goodla , Auemphon Mordmuang

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引用次数: 1

Abstract

This study aims to determine the effects of the extracts of Streblus asper, Cymbopogon citratus, Syzygium aromaticum and its formulation of green synthesized silver nanoparticle (AgNPs) on Streptococcus mutans growth and biofilm formation. The ethanolic extracts of S. asper, C. citratus, S. aromaticum, and a mix of the three herbs demonstrated antibacterial activity against S. mutans isolates by reducing bacterial biofilm formation and decreasing bacterial cell surface hydrophobicity. The formulated AgNPs from the ethanolic extracts could enhance the antibacterial activities of the plant extracts. Molecular docking found the best interaction between luteolin isolated from C. citratus and glucosyltransferase protein (GtfB), assuming the promising anti-biofilm activity. The scanning electron microscopy revealed morphological changes in the biofilm structure and a significant decrease in the biofilm area of the AgNPs treated. The study suggested that the extracts and its application could be used as natural alternative agents with multi-action against S. mutans infections.

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草药提取物及其绿色合成纳米颗粒制剂对变形链球菌相关生物膜的抗菌作用

本研究旨在确定Streblus asper、Cymbopogon citratus、Syzygium芳香草的提取物及其绿色合成银纳米粒子（AgNPs）配方对变形链球菌生长和生物膜形成的影响。S.asper、C.citratus、S.aromicum的乙醇提取物和三种草药的混合物通过减少细菌生物膜的形成和降低细菌细胞表面的疏水性，对变形链球菌分离株表现出抗菌活性。由乙醇提取物配制的AgNPs可以增强植物提取物的抗菌活性。分子对接发现，从柠檬曲霉中分离的木犀草素与葡萄糖基转移酶蛋白（GtfB）之间的相互作用最好，假设其具有良好的抗生物膜活性。扫描电子显微镜显示，经处理的AgNPs的生物膜结构发生了形态学变化，生物膜面积显著减少。研究表明，该提取物及其应用可作为天然替代剂，对变形链球菌感染具有多种作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology Reports Immunology and Microbiology-Applied Microbiology and Biotechnology

CiteScore

15.80

自引率

0.00%

发文量

审稿时长

55 days

期刊介绍： Biotechnology Reports covers all aspects of Biotechnology particularly those reports that are useful and informative and that will be of value to other researchers in related fields. Biotechnology Reports loves ground breaking science, but will also accept good science that can be of use to the biotechnology community. The journal maintains a high quality peer review where submissions are considered on the basis of scientific validity and technical quality. Acceptable paper types are research articles (short or full communications), methods, mini-reviews, and commentaries in the following areas: Healthcare and pharmaceutical biotechnology Agricultural and food biotechnology Environmental biotechnology Molecular biology, cell and tissue engineering and synthetic biology Industrial biotechnology, biofuels and bioenergy Nanobiotechnology Bioinformatics & systems biology New processes and products in biotechnology, bioprocess engineering.