Dietary medium-chain triglycerides upregulate metabolic gene expression in the gastrocnemius and downregulate expression of muscle-degradation gene in the soleus in aged bromodomain-containing 4+/- heterozygous mice

IF 2.4 Q3 NUTRITION & DIETETICS
Mayu Kimura , Shiori Ishiyama , Takashi Ando , Yuji Tanaka , Keiko Ozato , Kazuki Mochizuki
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Abstract

Purpose

Bromodomain containing 4 (BRD4) positively regulates metabolic gene expression, and Brd4+/- heterozygous mice exhibit leanness and malnutrition-like features. We evaluated gene expression changes, particularly of metabolism and muscle synthesis/degradation, in the skeletal muscles of aged Brd4+/- mice fed a high medium-chain triglyceride (MCT) diet.

Methods

Each organ weight of C57BL/6J male wild-type and Brd4+/-/C57BL/6J mice were assessed at approximately 100 weeks old. Male Brd4+/-/C57BL/6J mice (aged 54–84 weeks) were fed a control or MCT diet for approximately 5 months and mRNA expression changes in the gastrocnemius and soleus were quantified using mRNA microarray analysis and qRT-PCR.

Results

Gastrocnemius and soleus weights decreased markedly in aged Brd4+/-, compared to wild-type mice; MCT-fed Brd4+/- mice had decreased mesenteric adipose tissue weight but no decrease in gastrocnemius and soleus weights. Metabolic gene expression was downregulated more in the gastrocnemius than in the soleus. Metabolic genes, including glycolytic and citric acid cycle, and Myh3, mediating regeneration of the skeletal muscle, in gastrocnemius, were upregulated in MCT-diet-fed Brd4+/-, whereas Murf1, which promotes degradation, was downregulated in soleus.

Conclusions

An MCT-rich diet altered mRNA expression of metabolic and muscle-regeneration and -degradation genes in the gastrocnemius and soleus of Brd4+/- mice.

饮食中链甘油三酯上调老年含溴结构域4+/-杂合小鼠腓肠肌代谢基因表达,下调比目鱼肌肌肉降解基因表达
目的含溴4(BRD4)正调控代谢基因表达,BRD4+/-杂合小鼠表现出消瘦和营养不良样特征。我们评估了喂食高-中链甘油三酯(MCT)饮食的老年Brd4+/-小鼠骨骼肌中的基因表达变化,特别是代谢和肌肉合成/降解的变化。方法在大约100周龄时评估C57BL/6J雄性野生型和Brd4+/-/C57BL/6J小鼠的每个器官重量。雄性Brd4+/-C57BL/6J小鼠(54–84周龄)喂食对照或MCT饮食约5个月,并使用信使核糖核酸微阵列分析和qRT-PCR定量腓肠肌和比目鱼肌的信使核糖核酸表达变化。结果与野生型小鼠相比,老龄Brd4++-的腓肠肌和腓肠肌重量显著降低;MCT喂养的Brd4+/-小鼠肠系膜脂肪组织重量减少,但腓肠肌和比目鱼肌重量没有减少。代谢基因表达在腓肠肌中的下调幅度大于比目鱼肌。代谢基因,包括糖酵解和柠檬酸循环,以及介导腓肠肌骨骼肌再生的Myh3,在MCT饮食喂养的Brd4+/-中上调,而促进降解的Murf1在比目鱼肌中下调。结论富含MCT的饮食改变了Brd4+/-小鼠腓肠肌和比目鱼肌代谢、肌肉再生和降解基因的mRNA表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
PharmaNutrition
PharmaNutrition Agricultural and Biological Sciences-Food Science
CiteScore
5.70
自引率
3.10%
发文量
33
审稿时长
12 days
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