An electrochemical biosensor for the determination of folic acid in pregnant women based on DHFR/c-MWCNTs/TiO2NPs modified gold electrode

Sensors International Pub Date : 2023-01-01 DOI:10.1016/j.sintl.2023.100235

Bhawna Batra , Sarita Yadav , Vijay Kalra , Minakshi Sharma , J.S. Rana

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引用次数: 2

Abstract

The present research article describes a newly designed sensing method for determining folic acid levels. Folic acid is a B vitamin that helps the body makes healthy new cells. It is also known as vitamin B9 or folic acid. A new folic acid biosensor was constructed based on dihydrofolic acid reductase (DHFR) immobilized on c-MWCNT/TiO₂NPs modified gold electrode. Prepared titanium dioxide nanoparticles have been characterized by Transmission electron microscope (TEM), X-Ray Diffractometer (XRD), and Fourier Transform Infrared spectroscopy (FTIR). Fabricated working gold electrode (i.e. DHFR/c-MWCNTs/TiO₂NPs/AuE) was characterized at various stages of its construction by Scanning electron microscope (SEM), electrochemical impedance spectroscopy (EIS), FTIR, and cyclic voltammetry to confirm its fabrication. The biosensor showed an optimum response at pH 7.5 and 35 °C temperature. The maximum current of the constructed folic acid biosensor was due to electrons generated at 0.125 V against Ag/AgCl electrode. The proposed biosensor showed a low detection limit (11.48 nM), a wide linear range (5 nM–50 nM), a sensitivity of 0.42 μA/nM/cm², and good storage stability. Folic acid biosensor was evaluated and utilized for folic acid level quantification in serum samples of pregnant women.

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基于DHFR/c-MWCNTs/TiO2NPs修饰金电极的孕妇叶酸电化学生物传感器

本研究文章描述了一种新设计的测定叶酸水平的传感方法。叶酸是一种B族维生素，有助于身体产生健康的新细胞。它也被称为维生素B9或叶酸。基于固定化在c-MWCNT/TiO2NPs修饰金电极上的二氢叶酸还原酶（DHFR），构建了一种新型的叶酸生物传感器。用透射电子显微镜（TEM）、X射线衍射仪（XRD）和傅立叶变换红外光谱（FTIR）对制备的二氧化钛纳米粒子进行了表征。通过扫描电子显微镜（SEM）、电化学阻抗谱（EIS）、FTIR和循环伏安法对所制备的工作金电极（即DHFR/c-MWCNTs/TiO2NPs/AuE）在其构建的各个阶段进行了表征，以证实其制备。该生物传感器在pH 7.5和35时显示出最佳响应°C温度。构建的叶酸生物传感器的最大电流是由于在0.125下产生的电子V对抗Ag/AgCl电极。所提出的生物传感器显示出低检测极限（11.48nM），宽线性范围（5nM–50nM），灵敏度为0.42μA/nM/cm2，具有良好的储存稳定性。对叶酸生物传感器进行了评估，并将其用于孕妇血清样本中叶酸水平的定量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sensors International

CiteScore

17.40

自引率

0.00%

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