{"title":"HER2 targeted noninvasive immunosensor based on pencil graphite electrode for detection of breast cancer","authors":"Reeti Chaudhary , Bhawna Nohwal , Hitesh Bhardwaj , Deepa , C.S. Pundir","doi":"10.1016/j.sintl.2023.100238","DOIUrl":null,"url":null,"abstract":"<div><p>In the current research, we have fabricated an amperometric immunosensor for the detection of breast cancer using HER2 antibody. The sensor was fabricated by immobilization of anti-HER2 antibody on the surface of pencil graphite electrode (PGE). The techniques used for the characterization of the PGE/anti-HER2 were cyclic voltammetry, scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The sensor was optimized in terms of pH, temperature, antigen concentration, response time and antibody concentration. A linear range amidst 1 fgml<sup>−1</sup> and 20 ngml<sup>−1</sup> was observed. The limit of detection was 1 fg/ml. HER2 levels were also determined in sera samples of apparently healthy persons and breast cancer patients. The authentication of the fabricated immunosensor was noticeable due to its good regeneration ability and storage stability.</p></div>","PeriodicalId":21733,"journal":{"name":"Sensors International","volume":"4 ","pages":"Article 100238"},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensors International","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666351123000128","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
In the current research, we have fabricated an amperometric immunosensor for the detection of breast cancer using HER2 antibody. The sensor was fabricated by immobilization of anti-HER2 antibody on the surface of pencil graphite electrode (PGE). The techniques used for the characterization of the PGE/anti-HER2 were cyclic voltammetry, scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The sensor was optimized in terms of pH, temperature, antigen concentration, response time and antibody concentration. A linear range amidst 1 fgml−1 and 20 ngml−1 was observed. The limit of detection was 1 fg/ml. HER2 levels were also determined in sera samples of apparently healthy persons and breast cancer patients. The authentication of the fabricated immunosensor was noticeable due to its good regeneration ability and storage stability.
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