Limosilactobacillus reuteri inhibits the acid tolerance response in oral bacteria

IF 5.9 Q1 MICROBIOLOGY

Biofilm Pub Date : 2023-06-21 DOI:10.1016/j.bioflm.2023.100136

Gabriella Boisen , Zdenka Prgomet , Gabriela Enggren , Hanna Dahl , Cindy Mkadmi , Julia R. Davies

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引用次数: 1

Abstract

Probiotic bacteria show promising results in prevention of the biofilm-mediated disease caries, but the mechanisms are not fully understood. The acid tolerance response (ATR) allows biofilm bacteria to survive and metabolize at low pH resulting from microbial carbohydrate fermentation. We have studied the effect of probiotic strains: Limosilactobacillus reuteri and Lacticaseibacillus rhamnosus on ATR induction in common oral bacteria. Communities of L. reuteri ATCC PTA5289 and Streptoccus gordonii, Streptococcus oralis, Streptococcus mutans or Actinomyces naeslundii in the initial stages of biofilm formation were exposed to pH 5.5 to allow ATR induction, followed by a low pH challenge. Acid tolerance was evaluated as viable cells after staining with LIVE/DEAD®BacLight™. The presence of L. reuteri ATCC PTA5289 caused a significant reduction in acid tolerance in all strains except S. oralis. When S. mutans was used as a model organism to study the effects of additional probiotic strains (L. reuteri SD2112, L. reuteri DSM17938 or L. rhamnosus GG) as well as L. reuteri ATCC PTA5289 supernatant on ATR development, neither the other probiotic strains nor supernatants showed any effect. The presence of L. reuteri ATCC PTA5289 during ATR induction led to down-regulation of three key genes involved in tolerance of acid stress (luxS, brpA and ldh) in Streptococci. These data suggest that live cells of probiotic L. reuteri ATCC PTA5289 can interfere with ATR development in common oral bacteria and specific strains of L. reuteri may thus have a role in caries prevention by inhibiting development of an acid-tolerant biofilm microbiota.

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路氏乳杆菌抑制口腔细菌的耐酸反应

益生菌在预防生物膜介导的龋齿方面显示出良好的效果，但其机制尚不完全清楚。耐酸性反应（ATR）允许生物膜细菌在微生物碳水化合物发酵产生的低pH下生存和代谢。我们研究了益生菌菌株：路氏乳杆菌和鼠李糖乳杆菌对常见口腔细菌ATR诱导的影响。在生物膜形成的初始阶段，将路氏乳杆菌ATCC PTA5289和戈登链霉菌、口腔链球菌、变形链球菌或纳氏放线菌的群落暴露于pH 5.5以允许ATR诱导，然后进行低pH挑战。用LIVE/DEAD®BacLight染色后，将耐酸性评估为活细胞™. 路氏乳杆菌ATCC PTA5289的存在导致除口腔乳杆菌外的所有菌株的耐酸性显著降低。当变异链球菌用作模型生物来研究额外的益生菌菌株（路氏乳杆菌SD2112、路氏乳球菌DSM17938或鼠李糖乳杆菌GG）以及路氏乳霉菌ATCC PTA5289上清液对ATR发育的影响时，其他益生菌菌株和上清液均未显示出任何作用。路氏乳杆菌ATCC PTA5289在ATR诱导过程中的存在导致链球菌中参与酸应激耐受的三个关键基因（luxS、brpA和ldh）下调。这些数据表明，益生菌路氏乳杆菌ATCC PTA5289的活细胞可以干扰常见口腔细菌中的ATR发育，因此路氏乳球菌的特定菌株可以通过抑制耐酸生物膜微生物群的发育在防龋中发挥作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biofilm MICROBIOLOGY-

CiteScore

7.50

自引率

1.50%

发文量

审稿时长

57 days

期刊介绍：