Bacillus subtilis NDmed, a model strain for biofilm genetic studies

IF 5.9 Q1 MICROBIOLOGY
Yasmine Dergham , Dominique Le Coq , Arnaud Bridier , Pilar Sanchez-Vizuete , Hadi Jbara , Julien Deschamps , Kassem Hamze , Ken-ichi Yoshida , Marie-Françoise Noirot-Gros , Romain Briandet
{"title":"Bacillus subtilis NDmed, a model strain for biofilm genetic studies","authors":"Yasmine Dergham ,&nbsp;Dominique Le Coq ,&nbsp;Arnaud Bridier ,&nbsp;Pilar Sanchez-Vizuete ,&nbsp;Hadi Jbara ,&nbsp;Julien Deschamps ,&nbsp;Kassem Hamze ,&nbsp;Ken-ichi Yoshida ,&nbsp;Marie-Françoise Noirot-Gros ,&nbsp;Romain Briandet","doi":"10.1016/j.bioflm.2023.100152","DOIUrl":null,"url":null,"abstract":"<div><p>The <em>Bacillus subtilis</em> strain NDmed was isolated from an endoscope washer-disinfector in a medical environment. NDmed can form complex macrocolonies with highly wrinkled architectural structures on solid medium. In static liquid culture, it produces thick pellicles at the interface with air as well as remarkable highly protruding ‘‘beanstalk-like’’ submerged biofilm structures at the solid surface. Since these mucoid submerged structures are hyper-resistant to biocides, NDmed has the ability to protect pathogens embedded in mixed-species biofilms by sheltering them from the action of these agents. Additionally, this non-domesticated and highly biofilm forming strain has the propensity of being genetically manipulated. Due to all these properties, the NDmed strain becomes a valuable model for the study of <em>B</em>. <em>subtilis</em> biofilms. This review focuses on several studies performed with NDmed that have highlighted the sophisticated genetic dynamics at play during <em>B</em>. <em>subtilis</em> biofilm formation. Further studies in project using modern molecular tools of advanced technologies with this strain, will allow to deepen our knowledge on the emerging properties of multicellular bacterial life.</p></div>","PeriodicalId":55844,"journal":{"name":"Biofilm","volume":null,"pages":null},"PeriodicalIF":5.9000,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biofilm","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590207523000497","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 1

Abstract

The Bacillus subtilis strain NDmed was isolated from an endoscope washer-disinfector in a medical environment. NDmed can form complex macrocolonies with highly wrinkled architectural structures on solid medium. In static liquid culture, it produces thick pellicles at the interface with air as well as remarkable highly protruding ‘‘beanstalk-like’’ submerged biofilm structures at the solid surface. Since these mucoid submerged structures are hyper-resistant to biocides, NDmed has the ability to protect pathogens embedded in mixed-species biofilms by sheltering them from the action of these agents. Additionally, this non-domesticated and highly biofilm forming strain has the propensity of being genetically manipulated. Due to all these properties, the NDmed strain becomes a valuable model for the study of B. subtilis biofilms. This review focuses on several studies performed with NDmed that have highlighted the sophisticated genetic dynamics at play during B. subtilis biofilm formation. Further studies in project using modern molecular tools of advanced technologies with this strain, will allow to deepen our knowledge on the emerging properties of multicellular bacterial life.

枯草芽孢杆菌NDmed,生物膜遗传学研究的模式菌株
枯草芽孢杆菌NDmed菌株是在医疗环境中从内窥镜清洗消毒器中分离出来的。NDmed可以在固体介质上形成具有高度褶皱的建筑结构的复杂宏殖民地。在静态液体培养中,它在与空气的界面上产生厚的膜,并在固体表面产生显著的高度突出的“类eanstalk-like”浸没生物膜结构。由于这些粘液淹没的结构对杀生物剂具有超抗性,NDmed有能力通过保护嵌入混合物种生物膜中的病原体免受这些药剂的作用来保护它们。此外,这种非驯化且高度形成生物膜的菌株具有被基因操纵的倾向。由于所有这些特性,NDmed菌株成为研究枯草芽孢杆菌生物膜的有价值的模型。这篇综述的重点是用NDmed进行的几项研究,这些研究强调了枯草芽孢杆菌生物膜形成过程中复杂的遗传动力学。该项目使用先进技术的现代分子工具对该菌株进行进一步研究,将加深我们对多细胞细菌生命新特性的了解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Biofilm
Biofilm MICROBIOLOGY-
CiteScore
7.50
自引率
1.50%
发文量
30
审稿时长
57 days
期刊介绍:
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信