Combining phages and antibiotic to enhance antibiofilm efficacy against an in vitro dual species wound biofilm

IF 5.9 Q1 MICROBIOLOGY
Ergun Akturk , Luís D.R. Melo , Hugo Oliveira , Aurélie Crabbé , Tom Coenye , Joana Azeredo
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引用次数: 2

Abstract

Chronic wound management is extremely challenging because of the persistence of biofilm-forming pathogens, such as Pseudomonas aeruginosa and Staphylococcus aureus, which are the prevailing bacterial species that co-infect chronic wounds. Phage therapy has gained an increased interest to treat biofilm-associated infections, namely when combined with antibiotics. Here, we tested the effect of gentamicin as a co-adjuvant of phages in a dual species-biofilm wound model formed on artificial dermis. The biofilm-killing capacity of the tested treatments was significantly increased when phages were combined with gentamicin and applied multiple times as multiple dose (three doses, every 8 h). Our results suggest that gentamycin is an effective adjuvant of phage therapy particularly when applied simultaneously with phages and in three consecutive doses. The multiple and simultaneous dose treatment seems to be essential to avoid bacterial resistance development to each of the antimicrobial agents.

噬菌体和抗生素联合应用提高抗生物膜对体外双物种伤口生物膜的疗效
慢性伤口管理极具挑战性,因为形成生物膜的病原体持续存在,如铜绿假单胞菌和金黄色葡萄球菌,它们是共同感染慢性伤口的主要细菌。噬菌体疗法对治疗生物膜相关感染越来越感兴趣,即与抗生素联合使用。在这里,我们在人造真皮上形成的双物种生物膜伤口模型中测试了庆大霉素作为噬菌体的共同佐剂的作用。当噬菌体与庆大霉素联合应用并多次作为多剂量(每8小时三次)时,测试处理的生物膜杀伤能力显著提高。我们的研究结果表明,庆大霉素是噬菌体治疗的有效佐剂,尤其是当与噬菌体同时使用并连续三次使用时。多剂量和同时剂量治疗似乎对于避免细菌对每种抗菌剂产生耐药性至关重要。
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来源期刊
Biofilm
Biofilm MICROBIOLOGY-
CiteScore
7.50
自引率
1.50%
发文量
30
审稿时长
57 days
期刊介绍:
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