Glycosyl hydrolase from Pseudomonas fluorescens inhibits the biofilm formation of Pseudomonads

IF 5.9 Q1 MICROBIOLOGY
Di Wang , Syed Tatheer Alam Naqvi , Fanglin Lei , Zhenyu Zhang , Haiying Yu , Luyan Z. Ma
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引用次数: 0

Abstract

Biofilms are complex microbial communities embedded in extracellular matrix. Pathogens within the biofilm become more resistant to the antibiotics than planktonic counterparts. Novel strategies are required to encounter biofilms. Exopolysaccharides are one of the major components of biofilm matrix and play a vital role in biofilm architecture. In previous studies, a glycosyl hydrolase, PslGPA, from Pseudomonas aeruginosa was found to be able to inhibit biofilm formation by disintegrating exopolysaccharide in biofilms. Here, we investigate the potential spectrum of PslG homologous protein with anti-biofilm activity. One glycosyl hydrolase from Pseudomonas fluorescens, PslGPF, exhibits anti-biofilm activities and the key catalytic residues of PslGPF are conserved with those of PslGPA. PslGPF at concentrations as low as 50 nM efficiently inhibits the biofilm formation of P. aeruginosa and disassemble its preformed biofilm. Furthermore, PslGPF exhibits anti-biofilm activity on a series of Pseudomonads, including P. fluorescens, Pseudomonas stutzeri and Pseudomonas syringae pv. phaseolicola. PslGPF stays active under various temperatures. Our findings suggest that P. fluorescens glycosyl hydrolase PslGPF has potential to be a broad spectrum inhibitor on biofilm formation of a wide range of Pseudomonads.

荧光假单胞菌的糖基水解酶抑制假单胞菌生物膜的形成
生物膜是嵌入细胞外基质中的复杂微生物群落。生物膜内的病原体对抗生素的抵抗力比浮游生物更强。遇到生物膜需要新的策略。外多糖是生物膜基质的主要成分之一,在生物膜结构中起着至关重要的作用。在之前的研究中,来自铜绿假单胞菌的一种糖基水解酶PslGPA被发现能够通过分解生物膜中的胞外多糖来抑制生物膜的形成。在这里,我们研究了具有抗生物膜活性的PslG同源蛋白的潜在谱。荧光假单胞菌的一种糖基水解酶PslGPF具有抗生物膜活性,其关键催化残基与PslGPA的催化残基保守。低至50nM浓度的PslGPF有效抑制铜绿假单胞菌的生物膜形成并分解其预先形成的生物膜。此外,PslGPF对一系列假单胞菌表现出抗生物膜活性,包括荧光假单胞菌、stutzeri假单胞菌和丁香假单胞菌pv。Pharolicola。PslGPF在不同温度下保持活动。我们的研究结果表明,荧光假单胞菌糖基水解酶PslGPF有可能成为一种广谱的假单胞菌生物膜形成抑制剂。
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来源期刊
Biofilm
Biofilm MICROBIOLOGY-
CiteScore
7.50
自引率
1.50%
发文量
30
审稿时长
57 days
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