Dietary calcium (Ca2+) impacts Ca2+ content and molecular expression of Ca2+-transporters in Malpighian tubules of the yellow fever mosquito, Aedes aegypti
{"title":"Dietary calcium (Ca2+) impacts Ca2+ content and molecular expression of Ca2+-transporters in Malpighian tubules of the yellow fever mosquito, Aedes aegypti","authors":"Yuan Li, Peter M. Piermarini","doi":"10.1016/j.jinsphys.2023.104551","DOIUrl":null,"url":null,"abstract":"<div><p>The renal (Malpighian) tubules of insects play important roles in hemolymph Ca<sup>2+</sup> regulation. Here we investigated how dietary Ca<sup>2+</sup> loads from sucrose or blood meals affect the Ca<sup>2+</sup> content and mRNA expression of Ca<sup>2+</sup> transporters in Malpighian tubules of adult female mosquitoes. Using the yellow fever mosquito <em>Aedes aegypti</em> we found that feeding females 10% sucrose with elevated Ca<sup>2+</sup> concentration <em>ad libitum</em> for 6 days led to increased Ca<sup>2+</sup> content in Malpighian tubules. The increases of Ca<sup>2+</sup> content correlated with up-regulations of mRNAs encoding intracellular Ca<sup>2+</sup>-ATPases (<em>SERCA</em> and <em>SPCA</em>), a plasma membrane Ca<sup>2+</sup>-ATPase (<em>PMCA</em>), and a K<sup>+</sup>-dependent Na<sup>+</sup>/Ca<sup>2+</sup> exchanger (<em>NCKX1</em>). We also found that when adult females were fed blood, tubule Ca<sup>2+</sup> content changed dynamically over the next 72 h in a manner consistent with redistribution of tubule Ca<sup>2+</sup> stores to other tissues (e.g., ovaries). The changes in tubule Ca<sup>2+</sup> were correlated with dynamic changes in mRNA abundances of <em>SERCA</em>, <em>SPCA</em>, <em>PMCA</em>, and <em>NCKX1</em>. Our results are the first to demonstrate that Malpighian tubules of adult female mosquitoes have a remarkable capacity to handle high dietary Ca<sup>2+</sup> loads, most likely through the combination of storing excess Ca<sup>2+</sup> within intracellular compartments and secreting it into the tubule lumen for excretion. Our results also suggest that the Malpighian tubules play key roles in supplying Ca<sup>2+</sup> to other tissues during the processing of blood meals.</p></div>","PeriodicalId":16189,"journal":{"name":"Journal of insect physiology","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of insect physiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S002219102300077X","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The renal (Malpighian) tubules of insects play important roles in hemolymph Ca2+ regulation. Here we investigated how dietary Ca2+ loads from sucrose or blood meals affect the Ca2+ content and mRNA expression of Ca2+ transporters in Malpighian tubules of adult female mosquitoes. Using the yellow fever mosquito Aedes aegypti we found that feeding females 10% sucrose with elevated Ca2+ concentration ad libitum for 6 days led to increased Ca2+ content in Malpighian tubules. The increases of Ca2+ content correlated with up-regulations of mRNAs encoding intracellular Ca2+-ATPases (SERCA and SPCA), a plasma membrane Ca2+-ATPase (PMCA), and a K+-dependent Na+/Ca2+ exchanger (NCKX1). We also found that when adult females were fed blood, tubule Ca2+ content changed dynamically over the next 72 h in a manner consistent with redistribution of tubule Ca2+ stores to other tissues (e.g., ovaries). The changes in tubule Ca2+ were correlated with dynamic changes in mRNA abundances of SERCA, SPCA, PMCA, and NCKX1. Our results are the first to demonstrate that Malpighian tubules of adult female mosquitoes have a remarkable capacity to handle high dietary Ca2+ loads, most likely through the combination of storing excess Ca2+ within intracellular compartments and secreting it into the tubule lumen for excretion. Our results also suggest that the Malpighian tubules play key roles in supplying Ca2+ to other tissues during the processing of blood meals.
期刊介绍:
All aspects of insect physiology are published in this journal which will also accept papers on the physiology of other arthropods, if the referees consider the work to be of general interest. The coverage includes endocrinology (in relation to moulting, reproduction and metabolism), pheromones, neurobiology (cellular, integrative and developmental), physiological pharmacology, nutrition (food selection, digestion and absorption), homeostasis, excretion, reproduction and behaviour. Papers covering functional genomics and molecular approaches to physiological problems will also be included. Communications on structure and applied entomology can be published if the subject matter has an explicit bearing on the physiology of arthropods. Review articles and novel method papers are also welcomed.