Dietary calcium (Ca2+) impacts Ca2+ content and molecular expression of Ca2+-transporters in Malpighian tubules of the yellow fever mosquito, Aedes aegypti

IF 2.3 2区 农林科学 Q1 ENTOMOLOGY
Yuan Li, Peter M. Piermarini
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Abstract

The renal (Malpighian) tubules of insects play important roles in hemolymph Ca2+ regulation. Here we investigated how dietary Ca2+ loads from sucrose or blood meals affect the Ca2+ content and mRNA expression of Ca2+ transporters in Malpighian tubules of adult female mosquitoes. Using the yellow fever mosquito Aedes aegypti we found that feeding females 10% sucrose with elevated Ca2+ concentration ad libitum for 6 days led to increased Ca2+ content in Malpighian tubules. The increases of Ca2+ content correlated with up-regulations of mRNAs encoding intracellular Ca2+-ATPases (SERCA and SPCA), a plasma membrane Ca2+-ATPase (PMCA), and a K+-dependent Na+/Ca2+ exchanger (NCKX1). We also found that when adult females were fed blood, tubule Ca2+ content changed dynamically over the next 72 h in a manner consistent with redistribution of tubule Ca2+ stores to other tissues (e.g., ovaries). The changes in tubule Ca2+ were correlated with dynamic changes in mRNA abundances of SERCA, SPCA, PMCA, and NCKX1. Our results are the first to demonstrate that Malpighian tubules of adult female mosquitoes have a remarkable capacity to handle high dietary Ca2+ loads, most likely through the combination of storing excess Ca2+ within intracellular compartments and secreting it into the tubule lumen for excretion. Our results also suggest that the Malpighian tubules play key roles in supplying Ca2+ to other tissues during the processing of blood meals.

Abstract Image

日粮钙(Ca2+)对黄热病蚊马皮琴小管Ca2+含量和Ca2+转运蛋白分子表达的影响
昆虫的肾小管在血淋巴Ca2+调节中起着重要作用。在这里,我们研究了来自蔗糖或血餐的膳食Ca2+负荷如何影响成年雌性蚊子马尔皮氏管中Ca2+含量和Ca2+转运蛋白的mRNA表达。使用黄热病蚊子埃及伊蚊,我们发现随意喂食雌性10%蔗糖和升高的Ca2+浓度达6天,会导致Malpighian小管中Ca2+含量增加。Ca2+含量的增加与编码细胞内Ca2+-ATP酶(SERCA和SPCA)、质膜Ca2+-ATPase(PMCA)和K+依赖性Na+/Ca2+交换剂(NCKX1)的mRNA的上调有关。我们还发现,当成年雌性接受血液喂养时,小管Ca2+含量在接下来的72小时内动态变化,其方式与小管Ca2+储存向其他组织(如卵巢)的再分配一致。小管Ca2+的变化与SERCA、SPCA、PMCA和NCKX1的mRNA丰度的动态变化相关。我们的研究结果首次证明,成年雌性蚊子的马尔皮氏管具有显著的处理高膳食Ca2+负荷的能力,很可能是通过将过量的Ca2+储存在细胞内并分泌到小管腔中进行排泄的组合。我们的研究结果还表明,在血液膳食处理过程中,马尔皮氏管在向其他组织供应Ca2+方面发挥着关键作用。
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来源期刊
Journal of insect physiology
Journal of insect physiology 生物-昆虫学
CiteScore
4.50
自引率
4.50%
发文量
77
审稿时长
57 days
期刊介绍: All aspects of insect physiology are published in this journal which will also accept papers on the physiology of other arthropods, if the referees consider the work to be of general interest. The coverage includes endocrinology (in relation to moulting, reproduction and metabolism), pheromones, neurobiology (cellular, integrative and developmental), physiological pharmacology, nutrition (food selection, digestion and absorption), homeostasis, excretion, reproduction and behaviour. Papers covering functional genomics and molecular approaches to physiological problems will also be included. Communications on structure and applied entomology can be published if the subject matter has an explicit bearing on the physiology of arthropods. Review articles and novel method papers are also welcomed.
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