Applicability of Scoring Calyculin A-Induced Premature Chromosome Condensation Objects for Dose Assessment Including for Radiotherapy Patients.

IF 1.7 4区 生物学 Q4 CELL BIOLOGY
Cytogenetic and Genome Research Pub Date : 2023-01-01 Epub Date: 2023-10-25 DOI:10.1159/000534656
Mingzhu Sun, Jayne Moquet, David Lloyd, Stephen Barnard, Selvakumar Anbalagan, Harriet Steel, Aurore Sommer, Lone Gothard, Navita Somaiah, Elizabeth Ainsbury
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Abstract

As an extension to a previous study, a linear calibration curve covering doses from 0 to 10 Gy was constructed and evaluated in the present study using calyculin A-induced premature chromosome condensation (PCC) by scoring excess PCC objects. The main aim of this study was to assess the applicability of this PCC assay for doses below 2 Gy that are critical for triage categorization. Two separate blind tests involving a total of 6 doses were carried out; 4 out of 6 dose estimates were within the 95% confidence limits (95% CL) with the other 2 just outside. In addition, blood samples from five cancer patients undergoing external beam radiotherapy (RT) were also analyzed, and the results showed whole-body dose estimates statistically comparable to the dicentric chromosome assay (DCA) results. This is the first time that calyculin A-induced PCC was used to analyze clinical samples by scoring excess objects. Although dose estimates for the pre-RT patient samples were found to be significantly higher than the mean value for the healthy donors and were also significantly higher than those obtained using DCA, all these pre-treatment patients fell into the same category as those who may have received a low dose (<1 Gy) and do not require immediate medical care during emergency triage. Additionally, for radiological accidents with unknown exposure scenario, PCC objects and rings can be scored in parallel for the assessment of both low- and high-dose exposures. In conclusion, scoring excess objects using calyculin A-induced PCC is confirmed to be another potential biodosimetry tool in radiological emergency particularly in mass casualty scenarios, even though the data need to be interpreted with caution when cancer patients are among the casualties.

calyculin A诱导的过早染色体凝聚(PCC)对象评分在剂量评估(包括放疗患者)中的适用性。
作为先前研究的延伸,在本研究中,通过对过量的PCC对象进行评分,使用calyculin a诱导的过早染色体凝聚(PCC)构建并评估了覆盖0至10Gy剂量的线性校准曲线。本研究的主要目的是评估这种PCC测定法在2 Gy以下剂量下的适用性,这对分诊分类至关重要。进行了两次单独的盲测,共涉及6剂。6个剂量估计值中有4个在95%置信区间(95%CL)内,其他2个在95%可信区间外。此外,还分析了五名接受外照射放射治疗(RT)的癌症患者的血液样本,结果显示全身剂量估计值与双心室染色体分析(DCA)结果具有统计学可比性。这是首次使用calyculin A诱导的PCC通过对过量对象进行评分来分析临床样本。尽管发现RT前患者样本的剂量估计值显著高于健康供体的平均值,也显著高于使用DCA获得的剂量估计,但所有这些治疗前患者都与那些可能接受了低剂量(<1 Gy)且在紧急分诊期间不需要立即医疗护理的患者属于同一类。此外,对于暴露情况未知的放射性事故,可以对PCC物体和环进行平行评分,以评估低剂量和高剂量暴露。总之,使用calyculin A诱导的PCC对多余物体进行评分被证实是放射性紧急情况下的另一种潜在生物剂量测量工具,特别是在大规模伤亡情况下,尽管当癌症患者是伤亡者时,需要谨慎解释数据。
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来源期刊
Cytogenetic and Genome Research
Cytogenetic and Genome Research 生物-细胞生物学
CiteScore
3.10
自引率
5.90%
发文量
25
审稿时长
1 months
期刊介绍: During the last decades, ''Cytogenetic and Genome Research'' has been the leading forum for original reports and reviews in human and animal cytogenetics, including molecular, clinical and comparative cytogenetics. In recent years, most of its papers have centered on genome research, including gene cloning and sequencing, gene mapping, gene regulation and expression, cancer genetics, comparative genetics, gene linkage and related areas. The journal also publishes key papers on chromosome aberrations in somatic, meiotic and malignant cells. Its scope has expanded to include studies on invertebrate and plant cytogenetics and genomics. Also featured are the vast majority of the reports of the International Workshops on Human Chromosome Mapping, the reports of international human and animal chromosome nomenclature committees, and proceedings of the American and European cytogenetic conferences and other events. In addition to regular issues, the journal has been publishing since 2002 a series of topical issues on a broad variety of themes from cytogenetic and genome research.
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