Comparative cryopreservation of indian wild orange (Citrus indica Tanaka) embryonic axes.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2023-05-01

S K Malik, S Kaur, R Choudhary, R Chaudhury, H W Pritchard

{"title":"Comparative cryopreservation of indian wild orange (Citrus indica Tanaka) embryonic axes.","authors":"S K Malik, S Kaur, R Choudhary, R Chaudhury, H W Pritchard","doi":"","DOIUrl":null,"url":null,"abstract":"Background: Indian Wild Orange (Citrus indica Tanaka) is an endangered and endemic species from northeast India for which effective ex situ conservation strategies, including embryo cryopreservation, are urgently needed.Materials and methods: Desiccation tolerance and cryopreservation ability for embryonic axes of Citrus indica was determined using three techniques (air desiccation-freezing, PVS2 vitrification-freezing and encapsulation-dehydration-freezing). Success was assessed as survival and recovery in vitro.Results: Successful cryopreservation of embryonic axes was achieved using all three methods, with the highest survival achieved when using air desiccation-freezing (90%) followed by encapsulation-dehydration (85%) and PVS2 vitrification cryopreservation (80%). Regeneration levels were lower than survival levels for all three proceedures. Post-cryo regeneration success was: encapsulation-dehydration (64%) > air desiccation-freezing (55%) > PVS2 vitrification (52%).Conclusion: Although there was relatively high post-cryopreservation recovery growth obtained using all the three techniques, the air desiccation-freezing technique is preferred, as it is a simple, practical and reproducible technique for the long-term cryobanking of this important wild species. Doi: 10.54680/fr23310110512.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":"44 3","pages":"142-150"},"PeriodicalIF":1.0000,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cryo letters","FirstCategoryId":"99","ListUrlMain":"","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Indian Wild Orange (Citrus indica Tanaka) is an endangered and endemic species from northeast India for which effective ex situ conservation strategies, including embryo cryopreservation, are urgently needed.

Materials and methods: Desiccation tolerance and cryopreservation ability for embryonic axes of Citrus indica was determined using three techniques (air desiccation-freezing, PVS2 vitrification-freezing and encapsulation-dehydration-freezing). Success was assessed as survival and recovery in vitro.

Results: Successful cryopreservation of embryonic axes was achieved using all three methods, with the highest survival achieved when using air desiccation-freezing (90%) followed by encapsulation-dehydration (85%) and PVS2 vitrification cryopreservation (80%). Regeneration levels were lower than survival levels for all three proceedures. Post-cryo regeneration success was: encapsulation-dehydration (64%) > air desiccation-freezing (55%) > PVS2 vitrification (52%).

Conclusion: Although there was relatively high post-cryopreservation recovery growth obtained using all the three techniques, the air desiccation-freezing technique is preferred, as it is a simple, practical and reproducible technique for the long-term cryobanking of this important wild species. Doi: 10.54680/fr23310110512.

本刊更多论文

印度野橙（Citrus indica Tanaka）胚胎轴的比较冷冻保存。

背景：印度野橙（Citrus indica Tanaka）是印度东北部的一种濒危和特有物种，迫切需要有效的迁地保护策略，包括胚胎冷冻保存。材料与方法：采用空气干燥冷冻、PVS2玻璃化冷冻和包埋脱水冷冻三种技术，测定了柑桔胚轴的耐干燥性和冷冻保存能力。成功评估为体外存活和恢复。结果：三种方法都能成功冷冻保存胚胎轴，其中空气干燥冷冻保存的存活率最高（90%），其次是包埋脱水（85%）和PVS2玻璃化冷冻保存（80%）。所有三个过程的再生水平均低于存活水平。冷冻后再生成功率为：包封脱水（64%）>空气干燥冷冻（55%）>PVS2玻璃化（52%），用于长期冷冻保存这种重要野生物种的实用和可再生技术。Doi:10.54680/fr23310110512。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.