Assessing the benefits of antioxidant and antibiotics treatments for cryopreservation of the model alga Ectocarpus siliculosus and the endemic alga Acinetospora asiatica.

IF 1 4区 生物学 Q3 BIOLOGY
Cryo letters Pub Date : 2023-05-01
J Avila-Peltroche, B Y Won, T O Cho
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引用次数: 0

Abstract

Background: Cryopreservation in liquid nitrogen is a suitable technique for preserving seaweeds, a group of photosynthetic organisms with many applications. Although there are some standard protocols for seaweed cryopreservation, most rely on expensive controlled-rate coolers. Moreover, several factors, such as the use of antioxidants or antibiotics, remain unexplored.

Objective: To test the effect of 2-mercapthoethanol (antioxidant) and antibiotic mixtures on the cryopreservation of the model alga Ectocarpus siliculosus and the endemic brown seaweed Acinetospora asiatica using a low-tech passive rate cooler.

Materials and methods: 2-mercaptoethanol was added to the cryoprotectant (CPA) solution, while antibiotic mixtures were included in the culture medium during the recovery process. In addition, two CPA solutions were tested on E. siliculosus.

Results: After two weeks of recovery, the treatment comprising PSC antibiotic mixture (Penicillin G, streptomycin, and chloramphenicol) showed a significant increase in post-thaw viability. Antioxidant treatment did not improve viability. The highest viabilities for E. siliculosus and A. asiatica were 64-83%, and 83-87%, respectively, using 10% glycerol + 10% proline as CPA solution.

Conclusion: E. siliculosus and A. asiatica were successfully cryopreserved using a low-tech passive rate cooler, 10% glycerol + 10% proline solution, and antibiotic treatment. The highest post-thaw viabilities (64-87%) reported for PSC antibiotic mixture suggest the potential benefits of using antibiotics during post-thaw recovery of marine macroalgae. This study is the first report on the cryopreservation of A. asiatica. DOI: 10.54680/fr23310110212.

评估抗氧化剂和抗生素处理对模式藻类Ectocarpus siliculosus和地方藻类亚洲酸藻冷冻保存的益处。
背景:液氮冷冻保存是一种适合保存海藻的技术,海藻是一类具有多种应用的光合生物。尽管海藻冷冻保存有一些标准协议,但大多数都依赖于昂贵的控制速率冷却器。此外,一些因素,如抗氧化剂或抗生素的使用,仍未得到探索。目的:用低技术的被动降温装置,检测2-巯基乙醇(抗氧化剂)和抗生素混合物对模式藻类Ectocarpus siliculosus和地方性褐藻Acinetospora asiatica的冷冻效果。材料和方法:在冷冻保护剂(CPA)溶液中加入2-巯基乙醇,在回收过程中在培养基中加入抗生素混合物。此外,两种CPA溶液在E.siliculosus上进行了测试。结果:恢复两周后,包括PSC抗生素混合物(青霉素G、链霉素和氯霉素)的治疗显示解冻后的生存能力显著增加。抗氧化剂处理并没有提高生存能力。当使用10%甘油+10%脯氨酸作为CPA溶液时,C.siliculosus和A.asiatica的最高存活率分别为64-83%和83-87%。结论:采用低技术的被动降温器、10%甘油+10%脯氨酸溶液和抗生素处理,成功地冷冻保存了水杨和积雪草。PSC抗生素混合物的最高解冻后存活率(64-87%)表明,在海洋大型藻类的解冻后回收过程中使用抗生素具有潜在的好处。本研究首次报道了积雪草的冷冻保存。DOI:10.54680/fr23310110212。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Cryo letters
Cryo letters 生物-生理学
CiteScore
1.80
自引率
10.00%
发文量
50
审稿时长
1 months
期刊介绍: A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.
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