Human follicular fluid and mesenchymal stem cell conditioned medium improves in vitro development of vitrified-warmed mouse oocytes.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2023-03-01

S Geravandi, E Kalehoei, A Karami, F Nowrouzi, Z Kalhori, H Zhaleh, M Azadbakht

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引用次数: 0

Abstract

Background: In vitro maturation (IVM) and oocyte cryopreservation are therapeutic options in assisted reproductive technology which is used to preserve fertility in patients with different causes of infertility.

Objective: To analyze in vitro development of vitrified-warmed oocytes in the presence of human follicular fluid (FF) and bone marrow mesenchymal stem cell-conditioned medium (BMSC-CM) as a rescue strategy in fertility preservation.

Materials and methods: BMSC-CM and FF media were used as two natural media. Not only osteogenic and adipogenic differentiation but also flow cytometry was carried out to confirm the nature of mesenchymal stem cells. A total of 327 vitrified-warmed oocytes were randomly assigned to three groups with different maturation media. After 24 h the maturation rate was evaluated. In vitro fertilization and also embryo development were also assessed.

Results: Oocytes matured in the BMSC-CM and FF groups showed a significant increase compared to the control group (76.6+/-2.9, 53.2±1.0 , and 40.8+/-6.1, respectively) (P % 0.05). Embryo cleavage rates in the BMSC-CM were dramatically higher than FF and control groups (85.6+/-2.2, 70.5+/-2.2, and 60.7+/-1.5, respectively). Blastocyst formation rates in the BMSC-CM group were statically different compared to FF and control groups (73.6+/-1.0, 58.5+/-1.0, and 45.8+/-4.2, respectively).

Conclusion: BMSC-CM and FF media not only improve the maturation rate of vitrified warmed oocytes but also significantly increase embryo cleavage and blastocyst rates. DOI: 10.54680/fr23210110512.

本刊更多论文

人卵泡液和间充质干细胞条件培养基改善玻璃化加温小鼠卵母细胞的体外发育。

背景：体外成熟（IVM）和卵母细胞冷冻保存是辅助生殖技术中的治疗选择，用于保存不同原因不孕患者的生育能力。目的：分析在人卵泡液（FF）和骨髓间充质干细胞条件培养基（BMSC-CM）存在下玻璃化加温卵母细胞的体外发育，作为保存生育能力的挽救策略。材料和方法：采用BMSC-CM和FF两种天然培养基。不仅进行了成骨和成脂分化，还进行了流式细胞术来证实间充质干细胞的性质。共有327个玻璃化加温卵母细胞被随机分为三组，分别采用不同的成熟培养基。24小时后，评估成熟率。还对体外受精和胚胎发育进行了评估。结果：与对照组相比，BMSC-CM和FF组成熟的卵母细胞显著增加（分别为76.6+/-2.9、53.2±1.0和40.8+/-6.1）（P<0.05）。BMSC-CM组的胚胎切割率显著高于FF组和对照组（分别为85.6+/-2.2、70.5+/-2.2和60.7+/-1.5）。与FF组和对照组相比，BMSC-CM组的胚泡形成率有静态差异（分别为73.6+/-1.0、58.5+/-1.0和45.8+/-4.2）。DOI:10.54680/fr23210110512。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.