Biospecimen Qualification in a Clinical Biobank of Urological Diseases.

IF 1.6 4区生物学

Biopreservation and Biobanking Pub Date : 2024-06-01 Epub Date: 2023-10-25 DOI:10.1089/bio.2022.0190

Olga Kofanova, Sangita Paul, Achilleas Pexaras, Camille Bellora, Ala Petersons, Margaux Schmitt, Mohamad Baker Berjaoui, Yazan Qaoud, Miran Kenk, Heidi Wagner, Neil Fleshner, Fay Betsou

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引用次数: 0

Abstract

Development of novel biomarkers for diagnosis of disease and assessment of treatment efficacy utilizes a wide range of biospecimens for discovery research. The fitness of biospecimens for the purpose of biomarker development depends on the clinical characteristics of the donor and on a number of critical and potentially uncontrolled pre-analytical variables. Pre-analytical factors influence the reliability of the biomarkers to be analyzed and can seriously impact analytic outcomes. Sample quality stratification assays and tools can be utilized by biorepositories to minimize bias resulting from samples' inconsistent quality. In this study, we evaluated the quality of biobanked specimens by comparing analytical outcomes at 1, 5, and 10 years after collection. Our results demonstrate that currently available assays and tools can be used by biobank laboratories to support objective biospecimen qualification. We have established a workflow to monitor the quality of different types of biospecimens and, in this study, present the results of a qualification exercise applied to fluid samples and their derivatives in the context of urological diseases.

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泌尿系统疾病临床生物库的生物标本鉴定。

用于疾病诊断和治疗效果评估的新型生物标志物的开发利用了广泛的生物样本进行发现研究。生物样本用于生物标志物开发的适合性取决于供体的临床特征以及一些关键的和可能不受控制的预分析变量。预分析因素影响待分析生物标志物的可靠性，并可能严重影响分析结果。生物保藏中心可以利用样品质量分层分析和工具，最大限度地减少因样品质量不一致而产生的偏差。在这项研究中，我们通过比较采集后1年、5年和10年的分析结果来评估生物库标本的质量。我们的结果表明，生物库实验室可以使用目前可用的分析和工具来支持客观的生物样本鉴定。我们建立了一个工作流程来监测不同类型生物样本的质量，并在本研究中，介绍了在泌尿系统疾病背景下应用于流体样本及其衍生物的资格鉴定结果。

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来源期刊

Biopreservation and Biobanking Biochemistry, Genetics and Molecular Biology-General Biochemistry,Genetics and Molecular Biology

自引率

12.50%

发文量

114

期刊介绍： Biopreservation and Biobanking is the first journal to provide a unifying forum for the peer-reviewed communication of recent advances in the emerging and evolving field of biospecimen procurement, processing, preservation and banking, distribution, and use. The Journal publishes a range of original articles focusing on current challenges and problems in biopreservation, and advances in methods to address these issues related to the processing of macromolecules, cells, and tissues for research. In a new section dedicated to Emerging Markets and Technologies, the Journal highlights the emergence of new markets and technologies that are either adopting or disrupting the biobank framework as they imprint on society. The solutions presented here are anticipated to help drive innovation within the biobank community. Biopreservation and Biobanking also explores the ethical, legal, and societal considerations surrounding biobanking and biorepository operation. Ideas and practical solutions relevant to improved quality, efficiency, and sustainability of repositories, and relating to their management, operation and oversight are discussed as well.