Engineered live bacteria as disease detection and diagnosis tools.

IF 5.7 3区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Biological Engineering Pub Date : 2023-10-24 DOI:10.1186/s13036-023-00379-z

Imen Tanniche, Bahareh Behkam

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引用次数: 0

Abstract

Sensitive and minimally invasive medical diagnostics are essential to the early detection of diseases, monitoring their progression and response to treatment. Engineered bacteria as live sensors are being developed as a new class of biosensors for sensitive, robust, noninvasive, and in situ detection of disease onset at low cost. Akin to microrobotic systems, a combination of simple genetic rules, basic logic gates, and complex synthetic bioengineering principles are used to program bacterial vectors as living machines for detecting biomarkers of diseases, some of which cannot be detected with other sensing technologies. Bacterial whole-cell biosensors (BWCBs) can have wide-ranging functions from detection only, to detection and recording, to closed-loop detection-regulated treatment. In this review article, we first summarize the unique benefits of bacteria as living sensors. We then describe the different bacteria-based diagnosis approaches and provide examples of diagnosing various diseases and disorders. We also discuss the use of bacteria as imaging vectors for disease detection and image-guided surgery. We conclude by highlighting current challenges and opportunities for further exploration toward clinical translation of these bacteria-based systems.

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工程活菌作为疾病检测和诊断工具。

敏感和微创的医学诊断对于早期发现疾病、监测疾病进展和对治疗的反应至关重要。作为活传感器的工程细菌是一类新的生物传感器，用于以低成本对疾病发作进行灵敏、稳健、无创和原位检测。与微型机器人系统类似，简单的遗传规则、基本的逻辑门和复杂的合成生物工程原理的结合被用来将细菌载体编程为检测疾病生物标志物的活机器，其中一些是其他传感技术无法检测到的。细菌全细胞生物传感器（BWCB）可以具有广泛的功能，从仅检测到检测和记录，再到闭环检测调节治疗。在这篇综述文章中，我们首先总结了细菌作为活体传感器的独特优势。然后，我们描述了不同的基于细菌的诊断方法，并提供了诊断各种疾病和病症的例子。我们还讨论了细菌作为疾病检测和图像引导手术的成像载体的用途。最后，我们强调了当前的挑战和进一步探索这些基于细菌的系统的临床翻译的机会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Biological Engineering BIOCHEMICAL RESEARCH METHODS-BIOTECHNOLOGY & APPLIED MICROBIOLOGY

CiteScore

7.10

自引率

1.80%

发文量

审稿时长

17 weeks

期刊介绍： Biological engineering is an emerging discipline that encompasses engineering theory and practice connected to and derived from the science of biology, just as mechanical engineering and electrical engineering are rooted in physics and chemical engineering in chemistry. Topical areas include, but are not limited to: Synthetic biology and cellular design Biomolecular, cellular and tissue engineering Bioproduction and metabolic engineering Biosensors Ecological and environmental engineering Biological engineering education and the biodesign process As the official journal of the Institute of Biological Engineering, Journal of Biological Engineering provides a home for the continuum from biological information science, molecules and cells, product formation, wastes and remediation, and educational advances in curriculum content and pedagogy at the undergraduate and graduate-levels. Manuscripts should explore commonalities with other fields of application by providing some discussion of the broader context of the work and how it connects to other areas within the field.