Uncovering the heterogeneity and cell fate decisions of endothelial cells after myocardial infarction by single-cell sequencing

Medicine Advances Pub Date : 2023-09-26 DOI:10.1002/med4.34

Xinyang Long, Boteng Yan, Zengnan Mo

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Abstract

Background

The plasticity of endothelial cells (ECs) is crucial for tissue response to injury. Myocardial infarction can profoundly affect EC function, leading to a shift toward mesenchymal differentiation.

Methods

We utilized human single-nucleus RNA sequencing data to investigate the dynamic changes and cellular interactions between normal and post-infarction ECs.

Results

We identified two distinct subpopulations of ECs: A transient subpopulation characterized by short-term mesenchymal gene expression and a long-term subpopulation characterized by myocardial gene expression. Trajectory analysis revealed the differentiation pathways and potential roles over time and space. Furthermore, we uncovered the proliferation, differentiation, hypoxic, and inflammatory responses of ECs to injury.

Conclusions

Our study provides a comprehensive and detailed characterization of endothelial cell states, highlighting the role of activated endothelial cell subpopulations in promoting inflammation and tissue repair after infarction.

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单细胞测序揭示心肌梗死后内皮细胞的异质性和细胞命运决定

背景内皮细胞的可塑性对组织对损伤的反应至关重要。心肌梗死可严重影响EC功能，导致向间充质分化的转变。方法我们利用人类单核RNA测序数据来研究正常和梗死后内皮细胞之间的动态变化和细胞相互作用。结果我们确定了两个不同的内皮细胞亚群：以短期间充质基因表达为特征的短暂亚群和以心肌基因表达为特点的长期亚群。轨迹分析揭示了分化途径和在时间和空间上的潜在作用。此外，我们还揭示了内皮细胞对损伤的增殖、分化、缺氧和炎症反应。结论我们的研究提供了内皮细胞状态的全面而详细的表征，强调了活化的内皮细胞亚群在促进梗死后炎症和组织修复中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Medicine Advances

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