Tail regeneration in the gecko Sphaerodactylus argus shows that the formation of an axial elastic skeleton is functional for the new tail

IF 1.1 4区 生物学 Q4 ANATOMY & MORPHOLOGY
Acta Zoologica Pub Date : 2022-02-14 DOI:10.1111/azo.12416
Lorenzo Alibardi
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引用次数: 0

Abstract

Tail regeneration in the gecko Sphaerodactylus argus shows that the formation of an axial elastic skeleton is functional for the new tail (Acta Zoologica, Stockolm). The present autoradiographic and immunohistochemical study describes tail regeneration and formation of the axial skeleton in early regenerating tails of the Jamaican red-tailed gecko, Sphaerodactylus argus. Cell proliferation, studied by tritiated thymidine, shows intense labelling mainly in forming scales and differentiating cartilaginous, muscle and ependymal cells of the regenerating spinal cord, while the labelling is more diffuse in the apical blastema and proximal connective tissues. The slow apical proliferation maintains the tail front growing while in more proximal regions, cells initiate differentiation, losing thymidine-labelling. Cell proliferation is maximal at the beginning of scales, muscles and cartilage formation. Scales are regenerated following migration into the dermis of tritiated thymidine-labelled keratinocytes to form epithelial pegs that later split and give rise new scales. Differentiation of new corneous layers begins underneath the external corneous epidermis, starting with a shedding layer followed by a beta-layer that accumulates corneous beta proteins. Intense proliferation of apical myoblasts gives rise to long myotubes and segmented muscles. The vertebral column is substituted with a cartilaginous tube made of turgid chondrocytes accumulating chondroitin sulphate proteoglycan and elastin. Therefore, the regenerated tail remains flexible and capable of curling to maintain efficient the climbing ability in these geckos.

壁虎Spharodactylus argus的尾巴再生表明,轴向弹性骨架的形成对新尾巴具有功能
壁虎Spharodactylus argus的尾巴再生表明,轴向弹性骨架的形成对新尾巴具有功能(动物学报,Stockolm)。目前的放射自显影和免疫组织化学研究描述了牙买加红尾壁虎Spharodactylus argus的尾巴再生和早期再生尾巴轴向骨骼的形成。用氚化胸苷研究的细胞增殖显示出强烈的标记,主要在再生脊髓的软骨、肌肉和室管膜细胞的形成和分化中,而标记在顶端芽基和近端结缔组织中更为广泛。缓慢的顶端增殖维持了尾部前部的生长,而在更近的区域,细胞开始分化,失去胸苷标记。细胞增殖在鳞片、肌肉和软骨形成之初达到最大。鳞片在迁移到氚化胸苷标记的角质形成细胞的真皮中后再生,形成上皮钉,随后分裂并产生新的鳞片。新角质层的分化始于角质外表皮下方,首先是脱落层,然后是积累角质β蛋白的β层。顶端成肌细胞的强烈增殖产生长肌管和分节肌肉。用软骨管代替脊柱,软骨管由肿胀的软骨细胞组成,软骨细胞积聚硫酸软骨素蛋白多糖和弹性蛋白。因此,再生的尾巴保持灵活,能够卷曲,以保持这些壁虎有效的攀爬能力。
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来源期刊
Acta Zoologica
Acta Zoologica 生物-动物学
CiteScore
2.90
自引率
8.30%
发文量
35
审稿时长
>12 weeks
期刊介绍: Published regularly since 1920, Acta Zoologica has retained its position as one of the world''s leading journals in the field of animal organization, development, structure and function. Each issue publishes original research of interest to zoologists and physiologists worldwide, in the field of animal structure (from the cellular to the organismic level) and development with emphasis on functional, comparative and phylogenetic aspects. Occasional review articles are also published, as well as book reviews.
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