{"title":"Fluorescent RNA Tags for In Situ RNA Imaging in Living Cells","authors":"Peng Yin, Dr. Shi Kuang, Prof. Zhou Nie","doi":"10.1002/anse.202200090","DOIUrl":null,"url":null,"abstract":"<p>RNA imaging paves the way to investigate RNA function and regulation from the aspect of RNA subcellular localization and expression level. In the past decades, various highly precise and genetic coding fluorescent RNA, which can bind and light up the fluorophore by their specific sequence/structure, has been developed for RNA imaging, including Broccoli, Mango, SRB-2, RhoBAST, and Pepper et al. In this Review, we summarize the RNA imaging approaches and the progress made in the development of fluorescent RNA. Next, we emphasize the application of fluorescent RNA for in situ RNA imaging in living cells. Of particular note, besides the artificially selected fluorescent RNAs, a new kind of fluorophore that can bind and light up intrinsic RNA structure is described together for the first time. Finally, a summary of current strategies for designing fluorescent RNA and future perspectives are also presented. We hope this expanding topic will inspire the study of the architecture and functions of these fascinating molecular machinery in biological systems.</p>","PeriodicalId":72192,"journal":{"name":"Analysis & sensing","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analysis & sensing","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/anse.202200090","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
RNA imaging paves the way to investigate RNA function and regulation from the aspect of RNA subcellular localization and expression level. In the past decades, various highly precise and genetic coding fluorescent RNA, which can bind and light up the fluorophore by their specific sequence/structure, has been developed for RNA imaging, including Broccoli, Mango, SRB-2, RhoBAST, and Pepper et al. In this Review, we summarize the RNA imaging approaches and the progress made in the development of fluorescent RNA. Next, we emphasize the application of fluorescent RNA for in situ RNA imaging in living cells. Of particular note, besides the artificially selected fluorescent RNAs, a new kind of fluorophore that can bind and light up intrinsic RNA structure is described together for the first time. Finally, a summary of current strategies for designing fluorescent RNA and future perspectives are also presented. We hope this expanding topic will inspire the study of the architecture and functions of these fascinating molecular machinery in biological systems.
RNA成像为从RNA亚细胞定位和表达水平研究RNA的功能和调控铺平了道路。在过去的几十年里,各种高度精确和遗传编码的荧光RNA已经被开发用于RNA成像,包括Broccoli、Mango、SRB-2、RhoBAST和Pepper et 在这篇综述中,我们总结了RNA成像方法和荧光RNA的发展进展。接下来,我们强调荧光RNA在 活细胞中的原位RNA成像。特别值得注意的是,除了人工选择的荧光RNA外,还首次将一种能够结合并点亮固有RNA结构的新型荧光团结合在一起。最后,对目前荧光核糖核酸的设计策略进行了总结,并对未来进行了展望。我们希望这一不断扩展的主题将启发对生物系统中这些迷人分子机制的结构和功能的研究。