High prevalence of blaCTX-M-15 type extended-spectrum beta-lactamases in Gambian hooded vultures (Necrosyrtes monachus): A threatened species with substantial human interaction

IF 3.9 3区生物学 Q2 MICROBIOLOGY

MicrobiologyOpen Pub Date : 2023-03-23 DOI:10.1002/mbo3.1349

Hanna Woksepp, Fagimba Camara, Jonas Bonnedahl

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引用次数: 2

Abstract

One hundred fecal samples from hooded vultures in the Gambia (Banjul area) were investigated for the presence of bacteria with extended-spectrum cephalosporin- (ESBL/AmpC), carbapenemases, and colistin resistance. No Enterobacteriales carrying carbapenemases or resistance against colistin were detected. Fifty-four ESBL-producing Escherichia coli and five ESBL-producing Klebsiella pneumoniae isolates were identified in 52 of the samples, of which 52 E. coli and 4 K. pneumoniae yielded passed sequencing results. Fifty of the E. coli had ESBL phenotype and genotype harboring bla_CTX-M genes, of which 88.5% (n = 46) were the bla_CTX-M-15 gene, commonly found on the African continent. Furthermore, the genetic context around bla_CTX-M-15 was similar between isolates, being colocalized with ISKpn19. In contrast, cgMLST analysis of the E. coli harboring ESBL genes revealed a genetic distribution over a large fraction of the currently known existing E. coli populations in the Gambia. Hooded vultures in the Gambia thus have a high ESBL E. coli-prevalence (>50%) with low diversity regarding key resistance genes. Furthermore, given the urban presence and frequent interactions between hooded vultures and humans, data from this study implies hooded vultures as potential vectors contributing to the further dissemination of antibiotic-resistance genes.

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冈比亚秃鹫中blaCTX-M-15型超广谱β-内酰胺酶的高流行率：一种具有大量人类相互作用的濒危物种

对冈比亚（班珠尔地区）100只戴帽秃鹫的粪便样本进行了调查，以确定是否存在具有广谱头孢菌素（ESBL/AmpC）、碳青霉烯酶和粘菌素耐药性的细菌。未检测到携带碳青霉烯酶的肠杆菌或对粘菌素的耐药性。在52份样本中鉴定出54株产ESBL的大肠杆菌和5株产ESBL-肺炎克雷伯菌分离株，其中52株大肠杆菌和4株肺炎克雷伯菌测序结果合格。50株大肠杆菌具有ESBL表型和携带blaCTX-M基因的基因型，其中88.5%（n = 46）是常见于非洲大陆的blaCTX-M-15基因。此外，blaCTX-M-15周围的遗传环境在分离株之间是相似的，与ISKpn19共定位。相反，对携带ESBL基因的大肠杆菌的cgMLST分析显示，冈比亚目前已知的大肠杆菌种群中有很大一部分存在遗传分布。因此，冈比亚的带帽秃鹫具有较高的ESBL大肠杆菌流行率（>；50%），而关键抗性基因的多样性较低。此外，考虑到城市的存在以及戴帽秃鹫与人类之间的频繁互动，这项研究的数据表明，戴帽秃鹰是有助于抗生素耐药性基因进一步传播的潜在载体。

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来源期刊

MicrobiologyOpen MICROBIOLOGY-

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： MicrobiologyOpen is a peer reviewed, fully open access, broad-scope, and interdisciplinary journal delivering rapid decisions and fast publication of microbial science, a field which is undergoing a profound and exciting evolution in this post-genomic era. The journal aims to serve the research community by providing a vehicle for authors wishing to publish quality research in both fundamental and applied microbiology. Our goal is to publish articles that stimulate discussion and debate, as well as add to our knowledge base and further the understanding of microbial interactions and microbial processes. MicrobiologyOpen gives prompt and equal consideration to articles reporting theoretical, experimental, applied, and descriptive work in all aspects of bacteriology, virology, mycology and protistology, including, but not limited to: - agriculture - antimicrobial resistance - astrobiology - biochemistry - biotechnology - cell and molecular biology - clinical microbiology - computational, systems, and synthetic microbiology - environmental science - evolutionary biology, ecology, and systematics - food science and technology - genetics and genomics - geobiology and earth science - host-microbe interactions - infectious diseases - natural products discovery - pharmaceutical and medicinal chemistry - physiology - plant pathology - veterinary microbiology We will consider submissions across unicellular and cell-cluster organisms: prokaryotes (bacteria, archaea) and eukaryotes (fungi, protists, microalgae, lichens), as well as viruses and prions infecting or interacting with microorganisms, plants and animals, including genetic, biochemical, biophysical, bioinformatic and structural analyses. The journal features Original Articles (including full Research articles, Method articles, and Short Communications), Commentaries, Reviews, and Editorials. Original papers must report well-conducted research with conclusions supported by the data presented in the article. We also support confirmatory research and aim to work with authors to meet reviewer expectations. MicrobiologyOpen publishes articles submitted directly to the journal and those referred from other Wiley journals.

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